Epicatechin content in green tea (Camellia sinensis) supplements and their antioxidant activities

Introduction/background
Green tea produced from Camellia sinensis is a popular beverage and is consumed worldwide. Green tea produced from Camellia leaves are mainly consumed in East Asian countries including China, Japan and Taiwan; whereas in western parts and south Asian countries, black tea is relatively popular (Chan, et.al. 2011). Green tea produced from Camellia is believed to have several health benefits. The tea from Camellia mainly contains flavanols or catechins, epigallocatechins, epicatechin gallate and epicatechin. The tea is manufactured in such a way that the catechin oxidation by polyphenol oxidase is prevented (Chan, et.al. 2011). Although all of these components in green tea are reported to have several health benefits, in this study only the role of epicatechin in green tea from Camellia has been extensively reviewed. Epicatechin is a strong antioxidant, which has been suggested to have several health benefits (Ravindranath, et.al. 2009).
1.1. Project Aims

Considering the antioxidant activities of epicatechin content in Camellia sinensis, and having reviewed available literature on the subject area, this project therefore aims to;
Identify and quantify epicatechin content in herbal supplements by using standard compound.
Understand and evaluate the antioxidant property of the standard compound that has been reported to be the antioxidant activity of the supplements.
These aims were targeted with the views to providing clearer understanding of the role of antioxidants, present in green tea, as well as the health benefits associated to it.
1.2 Standardization of plant extracts
Herbal supplements are the complex mixtures containing organic chemicals derived from different plant sources including leaves, stems, flowers, roots and seeds. Although most herbal supplements and their compounds present in them are safe, many have been reported to have biologically active compounds that can alter several physiological processes within the body, as well as may interact with drugs. Thus, it is important to know the presence of active ingredients in the herbal supplement (Bent, 2008). Moreover, plant extracts are found to be rich in free radical and reactive oxygen species that
are implicated in the alterations of various metabolic processes, and may lead to
human diseases (Cases, et.al. 2010). Information on the biologically active compounds in herbal products will also help consumers in many aspects. Higher therapeutics effects can be seen in standardized extracts compared to the whole herb or non-standardized extracts; this is because, active principal is concentrated to a much higher level in the extracts than in the plant itself. Thus, purchasers of herbal products will get health benefits if extracts are standardized (Cases, et.al. 2010).
1.3 Antioxidants
Antioxidants are the compounds that are commonly derived from enzymes such as superoxide dismutase, catalase, and glutathione peroxidase and glutathione reductase; from minerals such as selenium, manganese, copper and zinc; as well as naturally from A, C and E vitamins (Lobo, et.al. 2010). Natural antioxidants are commonly found in plants consumed in the diet including carotenoids and phenolic compounds.
Moreover, plants contain high concentrations of numerous antioxidants that include polyphenols, carotenoids, tocopherols, tocotrienols, glutathione and ascorbic acid (Charles, 2013).
Their most important role in the body is to protect cells against the effects of free radicals. Free radicals are the unstable molecular species containing an unpaired electron that are able to exist independently. Most of these species are highly reactive and are potentially damaging to the cells (Lobo, et.al. 2010).
Some of the common oxygen- free radicals that are involved in many human diseases include hydroxyl radical, superoxide anion radical, hydrogen peroxide, oxygen singlet, hypochlorite, nitric oxide radical, and peroxynitrite radical. Increased levels of these reactive species in the body cells induce damage of the DNA, proteins, carbohydrates and lipids. Thus, a balance between free radicals and antioxidants is necessary for proper physiological function (Lobo, et.al. 2010). Any dis-balance in this may result the initiation of various diseases including cancer, vascular diseases and degenerative diseases. Hence, the study of antioxidants and their sources have been the focus of intense research.
1.4. Brief information on herbal supplements to use in the project
This project studies the presence of epicatechin content in Camellia sinensis; thus, the project uses herbal capsules from Camellia sinensis as herbal supplements. Camellia sinenis is taken as green tea and contains antioxidants in it, which is why it is commonly used in herbal medicine. Epicatechin is abundantly found in Camellia sinensis herbal supplement. Cameillia sinensis also contains various active compounds as extracts. Some of the major active compounds found in Camellia sinensis include catechins, caffeine, flavonols and proanthocyanidins (Charles, 2013). The health benefits of green tea (Camellia sinensis) epicatechins are being extensively studied and explained . Endothelial cells are associated to vascular function and homeostasis whose dysfunction may have implication in cardiovascular disease including atherogenesis. (Moore, et.al. 2009). Moreover, some studies suggest their anticancer role by enchaining apoptosis (Ravindranath, et.al. 2009). With findings emerging from several new studies regarding the health benefits of the green tea from Camellia sinensis, it is being increasingly popular among the people.
Methods
Simple and reliable techniques will be chosen in this project to investigate epicatechin content in Camellia sinenis. Experiments will be carried out using reflux extraction to determine the biologically active compounds present in the test supplement (herbal capsules from Camellia sinensis). This will be confirmed by NMR analysis. NMR analysis will be done to confirm that determination of correct biological compound including antioxidants from Camellia sinensis. Crude extract will be made from the material extracted from herbal capsules using a suitable solvent under reflux, which will then be run on thin layer chromatography (TLC) to see if a corresponding band for the standard also appears in the extract. This will tell whether or not the compound is present in the extract, as the study of organic compounds present in the supplement of Camellia sinensis can be made using this technique. To cut of the evaporation time, rotary evaporation method will be employed while evaporating the solvent. Reverse phase analytical high performance liquid chromatography (HPLC) will be used to identify the standard compound in the extract and then to quantify the amount of compound in it. Finally, the antioxidant assay will be carried out to determine the antioxidant activity of the extracted compound; as a control, plant Flavonoid quercetin, which has antioxidant activity, will be used.
REFERENCES
Charles, J.D. (2013). Antioxidant Properties of Spices, Herbs and Other Sources. Natural Antioxidants. 39-64.
Chan, E.W.C, Eu, Y.S, Tie, P.P, Law, Y.P. (2011). Pharmacognosy Research. Antioxidant and antibacterial properties of green, black, and herbal teas of Camellia sinensis. 3(4), 266-272.
Irshad, M and Chaudhuri, P.S. (2002). Indian journal of experimental biology. Oxidant-antioxidant system: role and significance in human body.. 40(11), 1233-1239.
Lobo, V, Patil, A, Phatak, A, Chandra, N. (2010). Pharmacogn Rev. Free radicals, antioxidants and functional foods: Impact on human health. 4(8), 118-126.
Moore, R.J, Jackson, K.G, Minihane, A.M. (2010). The British journal of nutrition. Green tea (Camellia sinensis) catechins and vascular function. 96(4), 597-605.
Ravindranath, M.H, et.al.,. (2006). Evid Based Complement Alternat Med.. Epicatechins Purified from Green Tea (Camellia sinensis) Differentially Suppress Growth of Gender-Dependent Human Cancer Cell Lines. 3(2), 237-247.
Ravindranath, et.al., (2009). Evidence-based complementary and alternative medicine. Differential Growth Suppression of Human Melanoma Cells by Tea (Camellia sinensis) Epicatechins (ECG, EGC and EGCG). 6(4), 523-530.
Roman, M, (2001) Natural Products Insider, http://www.naturalproductsinsider.com/articles/2001/04/the-benefits-and-pitfalls-of-standardizing-botanic.aspx, 11/12/2013.
Uzunalic, A.P, et.al.. (2006). Food Chemistry. Extraction of active ingredients from green tea (Camellia sinensis): Extraction efficiency of major catechins and caffeine. 96(4), 597-605.
Yang, Z, Xu, Y, Jie, G, He, P and Tu, Y. (2007). Asia Pac J Clin Nutr. Study on the antioxidant activity of tea flowers (Camellia sinensis) . 16(1), 148-152.

Calculate the price
Make an order in advance and get the best price
Pages (550 words)
$0.00
*Price with a welcome 15% discount applied.
Pro tip: If you want to save more money and pay the lowest price, you need to set a more extended deadline.
We know how difficult it is to be a student these days. That's why our prices are one of the most affordable on the market, and there are no hidden fees.

Instead, we offer bonuses, discounts, and free services to make your experience outstanding.
How it works
Receive a 100% original paper that will pass Turnitin from a top essay writing service
step 1
Upload your instructions
Fill out the order form and provide paper details. You can even attach screenshots or add additional instructions later. If something is not clear or missing, the writer will contact you for clarification.
Pro service tips
How to get the most out of your experience with MyhomeworkGeeks
One writer throughout the entire course
If you like the writer, you can hire them again. Just copy & paste their ID on the order form ("Preferred Writer's ID" field). This way, your vocabulary will be uniform, and the writer will be aware of your needs.
The same paper from different writers
You can order essay or any other work from two different writers to choose the best one or give another version to a friend. This can be done through the add-on "Same paper from another writer."
Copy of sources used by the writer
Our college essay writers work with ScienceDirect and other databases. They can send you articles or materials used in PDF or through screenshots. Just tick the "Copy of sources" field on the order form.
Testimonials
See why 20k+ students have chosen us as their sole writing assistance provider
Check out the latest reviews and opinions submitted by real customers worldwide and make an informed decision.
FIN571
excellent work
Customer 452773, March 1st, 2024
Business and administrative studies
Thank you for your hard work
Customer 452773, October 19th, 2023
ACC543MANAGERIALACCOUNTINGANDLEGALASPECTS
excellent
Customer 452773, January 25th, 2024
Business and administrative studies
excellent, got a 100
Customer 452773, May 17th, 2023
Leadership Studies
excellent job
Customer 452773, August 26th, 2023
BUSINESS LAW
excellent job made a 93
Customer 452773, March 22nd, 2023
Social Work and Human Services
Great work I would love to continue working with this writer thought out the 11 week course.
Customer 452667, May 30th, 2021
Business and administrative studies
looks good thank you
Customer 452773, March 3rd, 2023
10th grade English
very good
Customer 452773, March 26th, 2023
Business and administrative studies
excellent work
Customer 452773, March 12th, 2023
Business and administrative studies
Thanks
Customer 452773, March 3rd, 2023
Business and administrative studies
excellent paper
Customer 452773, March 3rd, 2023
11,595
Customer reviews in total
96%
Current satisfaction rate
3 pages
Average paper length
37%
Customers referred by a friend
OUR GIFT TO YOU
15% OFF your first order
Use a coupon FIRST15 and enjoy expert help with any task at the most affordable price.
Claim my 15% OFF Order in Chat
Close

Sometimes it is hard to do all the work on your own

Let us help you get a good grade on your paper. Get professional help and free up your time for more important courses. Let us handle your;

  • Dissertations and Thesis
  • Essays
  • All Assignments

  • Research papers
  • Terms Papers
  • Online Classes
Live ChatWhatsApp