GLUT1, found in the plasma membrane of erythrocytes, is a classic example of a uniporter. a….

 GLUT1, found in the plasma membrane of erythrocytes, is a classic example of a uniporter.

a. Design a set of experiments to prove that GLUT1 is indeed a glucose-specific uniporter rather than a galactoseor mannose-specific uniporter.

 b. Glucose is a six-carbon sugar, and ribose is a five-carbon sugar. Despite its smaller size, ribose is not efficiently transported by GLUT1. How can this be explained?

c. A drop in blood sugar from 5 mM to 2.8 mM or below can cause confusion and fainting. Calculate the effect of this drop on glucose transport into cells expressing GLUT1.

d. How do liver and muscle cells maximize glucose uptake without changing Vmax?

e. Tumor cells expressing GLUT1 often have a higher Vmax for glucose transport than do normal cells of the same type. How could these cells increase the Vmax?

 f. Fat and muscle cells modulate the Vmax for glucose uptake in response to insulin signaling. How?

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