Stress

Introduction

The hippocampus is a vital region of the brain that regulates major aspects of learning, memory and emotions. The hippocampus has also been linked in the regulation and control of anxiety response and conditioned fear (Yee et al. 2007) and yet it can also retain a high degree of plasticity (McEwen, Gianaros 2010). Looking at the structure of the hippocampus, one would find a curved arrow on either hemisphere. The curved region is known as the CA1-CA3 subfields which contain the pyramidal layer while the arrow known as the dentate gyrus (DG) is made up of a granular cell layer. The neurons in CA3 (the lower part of the curve) connects directly to CA1 (the upper part of the curve), which would receive input from the DG.

Another component of the hippocampus is the brain-deprived neurotrophic factor (BDNF) and its specific receptor TrkB. It has been found that in previous research that there is a high level of BDNF expression in the central nervous system in which it plays an important role in the survival, protection, maintenance and differentiation from insults to neural cells (Barde, 1989). In the hippocampus however, BDNF helps with long-term potentiation (Figurov, 1996). The majority of the neural effects of BDNF are regulated by binding to the TrkB receptor (Barde, 1989). The BDNF and TrkB could also play a role in the stress response in hippampal neurons (Tapia-Arancibia et al. 2004, Pardon et al. 2005, Sirianni et al. 2010).

Previous research has observed how BDNF and TrkB in the hippocampus play a role in the regulation of the HPA axis. Its role has to do with the termination of hypothalamopituitary-adrenocortical (HPA) axis responses to stress (Pizarro et al. 2004, McCormick et al. 2010), essentially the regulating the stress circuit to help maintain homeostasis (Herman et al. 1995). Other studies have found that this structure regulates the HPA axis through inhibiting its activity of BDNF and TrkB (Herman et al. 1996) and that hippocampal neurons can be hypersensitive to stress (Rot et al. 2009). They are especially vulnerable to long-term damaging influence of the glucocorticoids and can decrease expressions of BDNF (McEwen and Magarinos 2001, Bartolomucci et al. 2002, Murakami et al. 2005). It has been found that the consequences of long-term exposure to stress in the hippocampus can remodel hippocampal cells, which then can result in a malfunction of the affected area (Diamond et al.1996, Bremner 1999). Different types of chronic or acute stress can modulate the reduction of BDNF and TrkB mRNA expression in the hypothalamus and in the pituitary gland of adult male rats (Rage 2002, Murakami 2005, Givalois 2001).

Stress can play a more damaging role in the hippocampal regions of young and aged rats as well. Hippocampal vulnerability and reduction in neurotrophic factors have also been found in stressed and aging rats (Smith 1996, Li Yi 2009), and as well as in age-dependent rats that were postnatally exposed to maternal deprivation, which changed the BDNF expression in selected rat brain regions (Roceri, 2004). All of which can possibly affect the structural synaptic plasticity which is has been found to be preserved in the dentate gyrus of aged rats (Geinisman, 1992) and has been linked to certain mood disorders (Duman, 2000).

Many previous studies on the role of BDNF and TrkB in the hippocampus under certain stress conditions relied mainly on the observations of modifications in the matter of these proteins in adult animals during immobilization stress (Givalois et al. 2001, Rage et al. 2002, Marmigere et al. 2003, Reagan 2007). All of which has found a difference in the expression of BDNF in certain regions of the HPA axis in adult male rats, which again can lead to the dysfunction of that affected region. Furthermore, to my knowledge no reports have been published about chronic immobilization stress-induced responsiveness of BDNF and TrkB and their role in juvenile and aged animals exposed to immobilization stress during two critical stages of brain morphologic and functional transformations. The hypothesis of the proposed study is that juvenile rats will have a reduction of the hippocampal region than those of aged rats when exposed to chronic immobilization stress.

Methods

Animal

Twenty male Sprague-Dawley rats from two age groups would be used for this study. The first group described as juvenile –JUV (postnatal 28 days) and the second group described as aged –AGE (postnatal 360 days). They would be housed in groups of five animals per plastic cage in a room maintained under standardized light (8am to 8pm-hour light-dark cycle) and temperature (22±3°C) conditions. They would be housed at least 1 week prior to the experiment. The animals would receive free access to food pellets and tap water. The care and treatment of the rats would be in accordance with the guidelines for laboratory animals established by the National Institute of Health as well as by the Local Ethical Committee of the University of Massachusetts Amherst.

Experimental Procedure

All tests were conducted once a day in 60-minute sessions for 20 consecutive days at the same time, between 10:00am and 3:00pm. JUV and ADT animals would be divided into experimental groups (n=5), exposed to chronic immobilization stress and control non-stressed groups (n=5), which would remain in their home cages until perfusion. The control animals would be handled for a few minutes daily by the same operator. The chronic stress stimulation would begin for JUV rats at postnatal seven days old.
Chronic Immobilization Stress
During each session, the animals would be immobilized in accordance with a well-established protocol (Badowska-Szalewska, 2010). Rats are fixed on a wooden board (18?25 cm) in a supine position by means of a leather belt, after which each of their legs was fixed at an angle of 45° to the body midline with adhesive tape (Badowska-Szalewska, 2010). Experimental and control animals were sacrificed on postnatal day 28 (JUV) and 360 (ADT), 90 min after the final session. All animals will be deeply anesthetized with a lethal dose of a drug (choice pending) and then perfuse with 0.9% saline solution with heparin, followed by 4% paraformaldehyde solution in 0.1 M phosphate buffer (pH 7.4) (Badowska-Szalewska, 2010). The brains will be removed and kept overnight and serial coronal sections of brain (40-?m-thick) will be cut.

Immunohistochemistry

Bordering sections would be processed for BDNF and TrkB with immunohistochemistry. The free-floating sections will be blocked in 10% Normal Goat Serum (NGS) for 2 hours and incubated at 4?C for 3 days with the primary polyclonal rabbit anti-BDNF antibody and primary polyclonal rabbit anti-TrkB antibody (Badowska-Szalewska, 2010). After multiple rinses in a buffered saline (TBS), the sections will be incubated (2-3 hours, room temperature) with a secondary antibody. The controls for the immunohistochemical procedures will be processed with the same procedure with the exception of the primary or secondary antibodies. Therefore, no staining will be observed in the control slides.

Qualitative Analysis

An image analysis system will be used to analyze the number of BDNF-ir and TrkB-ir positive cells in the hippocampus. The total number will be divided into three (counted separately) areas of the hippocampus: CA1 subfield, CA3 subfield and dentate gyrus (DG). The cells that will be counted are the BDNF-ir cells or TrkB-ir cells in the pyramidal layer of CA1 and CA3, and in the granular layer of DG. The hemisphere and sampling will be chosen at random. The data will be analyzed by a two-way analysis of variance (ANOVA) for the factor groups (intact vs. chronic immobilization stress) and age groups (juvenile-JUV and aged-AGE).

Expected Results

Under chronic immobilization stress exposure, juvenile rats should have a significant decrease in the density of BDNF immunoreactive (ir) neurons and TrkB-ir cells should be observed in CA1, CA3 and DG. After chronic immobilization stress exposure of aged rats, the density of BDNF-ir and TrkB-ir cells should not decline in any of the sub-regions of the hippocampus.

Discussion

The present study would investigate the age-related changes in the density of BDNF-ir and TrkB-ir neurons in the under the exposure of immobilized stress. Prolonged forced swim has been found to affect the amount of BDNF-ir and TrkB-ir cells in the hippocampus of juvenile, not aged rats (Badowska 2010). The number of cells that contains these proteins would be higher in DG than in CA1 or CA3, which is mostly to be related to the various intrinsic and extrinsic interactions of the denrate gyrus (Amaral and Witter 1989). If proven, then the high levels of BDNF and TrkB that are present in the hippocampus indicate that these molecules have important physiological functions in different stages of life (Tapia-Arancibia et al. 2008). It is extremely important as demonstrated during adolescence; BDNF influences almost all aspects of development, including stimulation of growth, differentiation of neuronal stem cells and many other various roles (Mattson et al. 2004, Tapia-Arancibia et al. 2004). The flip side of the coin is that during aging, BDNF may play a protective role by preventing neurodegeneration, and stimulating sprouting in the hippocampus (Smith 1996, Tapia-Arancibia et al. 2004), or increasing neuronal repair (Smith et al. 1995).

As for TrkB, it exerts positive influence on dendritic branching and dendrtic integrity/plasicity in the hippocampus (Sato et al. 2001), therefore a reduction in the level of this protein may be underlying factor in the synaptic changes that occur with age in the hippocampus (Geinisman et al. 1992). It is also known that adolescent rats, versus adult subjects, tend to be more susceptible to the influence of aversive stimuli, which is created by HPA axis activation (Avital and Richter-Levin 2005, Lupien 2009), by the prolonged secretion of glucocorticoid (Romeo et al. 2004, Cruz et al. 2008, McCormick et al. 2010). Also the expression of BDNF and TrkB is possibly regulated in opposite direction, meaning that the growth of BDNF content occurs with the fall in the level of TrkB in the hippocampal cells of aged rats (Frank et al. 1997, Nibuya et al. 1999, Sommerfeld et al. 2000, Silhol M et al. 2007, Tapia-Arancibia et al. 2008).

Therefore, the higher density of BDNF-ir and the lower density of TrkB-ir cells in AGE group of experimental rats may signify their protective effects against hippocampal damage of aging animals in stress conditions. This study will demonstrate that hippocampal subfields of juvenile and aged rats show different density of BDNF and TrkB immunostaining cells. Chronic immobilization stress would influence the density of BDNF-ir and TrkB-ir in juvenile animals and the aged rats would be the determining factor in the changes in the density of BDNF-ir and TrkB-ir in the hippocampal regions.

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Eustress is your Friend Not all stress is bad. There are positive effects of stress and it has its own unique characteristics. When it comes to feelings such as anxiety, worry, fear, pain or anger then these are linked to negative stress and this is what many people associate stress with. However, the positive effects of stress are something that can help motivate individuals to accomplish something good. Positive stress helps an individual to make any necessary changes in their lives. So therefore, in my own personal opinion, stress is not such a bad thing. According to thefreedictionary. om, eustress by definition is a positive form of stress. Eustress is a balance between selfishness and altruism through which an individual develops the drive and energy to care for others. This refers to the constructive stress that helps keep you motivated and driven in all aspects of your life. For example, positive stress gives us a feeling of excitement when presented with a demanding situation in an area in which we are comfortable like runners on the track, taking a ride on the roller coaster, taking up a challenging project at workplace. Positive stress may fill you with energy to tackle the demands of your task.

Positive stress helps to keep us healthy and fills us with contentment. Eustress also reminds us that we can view many of the stressors in our lives as ‘challenges’ rather than ‘threats’, and have extra vital energy to handle these stressors, without a feeling of being overwhelmed or unhappy. There are many positive effects of stress including the ability to realize when things are wrong and need to be fixed. Positive stress also helps our body tell us when we need to do things such as put a sweater on when it is too cold, scratch an itch, move to a new location when necessary and other needs of everyday life.

Positive stress is also an excellent way of initiating the invention and problem solving process by providing more creative solutions. As a result positive stress has been linked to many scientific discoveries, technological advances and creative designs. Positive stress can also help us to know when we need medical care by altering us to when we are experiencing discomfort or other medical concerns. There are many examples of positive stressors. College students face a major stressor at the end of semesters. For instance, Megan is a freshman in a local community college.

She is the first in her family to attend school at this level. Her schedule is overwhelming but it is to be expected in order to obtain an associate’s degree. The semester is quickly closing in and Megan is having difficulties with her math class. She is already maintaining a C average and in order to pass, she must make an A on her final. Megan knows that she is feeling stress about this final but realizes that she needs to buckle down and concentrate. Approaching exams enhance a student’s concentration and learning capability. Therefore, this stress she is feeling is considered a ‘eustress. If Megan is able to obtain an A on this final, she will pass the class. But in passing the class, Megan will also be able to advance into the next math needed to obtain her degree. In the end, the stressors that Megan is dealing with will have positive effects on her life and future. Stay-at-home moms may seem to have an easy job, but in reality, it’s a wonderful job that includes quite a bit of stress. Many people may think that the life of a stay-at-home mom is all Everyone has their own personal opinion in regards to this statement, ‘Stress is always bad. I feel that stress always begins as positive stress. You just have to be willing to give your best and try to step up to the challenge. Almost all of the life’s important moments are experienced under positive stress. Your positive stress can turn to negative, but you must be willing to accept responsibility for the role you play in creating or maintaining that stress. So in my own personal opinion, I would have to disagree. Stress is only considered bad if you handle and cope with it badly. In the end, stress isn’t always bad.

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Emerging surveies further reveal that persons who initiate substance usage before 15 old ages of age take an norm of 29 old ages to accomplish one twelvemonth free of substances ( vs. 18 old ages for those who start utilizing after 20 old ages of age ) . In consonant rhyme with the above, the existent quandary of substance maltreatment stems from the fact that mistreating young persons frequently display co-occurring mental wellness issues ( Kim & A ; Jackson, 2009 ) . The early intercessions with those who initiate substance usage during adolescent old ages remain a lost chance for many persons ( Liddle, Rowe, Dakof, Henderson, & A ; Greenbaum, 2009 ) .

Eitle ( 2006 ) noted that populating in single-parent families peculiarly the male parent predicted increased marihuanas use among Hipic/Latinos, but non among African Americans or Whites. Harmonizing to Wagner, Olson, Chou, Pokhrel and Duan, et Al ( 2010 ) , the features of the household such as its operation and construction may play both protective and worsening functions in adolescent substance usage. The hazard factors for early stripling substance maltreatment have been identified ( Hawkins, Catalano, & A ; Miller, 1992 ) , and utilized by research workers to develop intercessions aiming vulnerable striplings ( Dishion, Kavanagh, Schneiger, Nelson, & A ; Kaufman, 2002 ) .

However spheres which may chair or intercede the consequence of substance maltreatment on adolescent substance maltreaters remains grossly under researched hence the demand for the present survey on the relationship between self-pride, emphasis, equal relationship, depression, household background and substance maltreatment among striplings. The term substance maltreatment was defined as a unidirectional concept. In kernel the term captures substance maltreatment as one concept, though multi-directional positions exist.

Background of the Study

Experts in developmental epidemiology of substance maltreatment suggest that substance usage during adolescence disrupts necessary adolescent developmental procedures ( Liddle, Rowe, Dakof, Henderson, & A ; Greenbaum, 2009 ) thereby easing the divergence from protective influences, such as the household and the school. Scholars every bit contend that it leads to the acceptance of a configuration of aberrant attitudes, activities, associations, and behaviours ( Okoza, Aluede, Fajoju, & A ; Okhiku, 2009 ; Flory, Lynam, Milich, Leukefeld, & A ; Clayton, 2004 ; Lynskey et al. , 2003 ) .

Surveies conducted by the Indiana Preventive Resource Center ( 2003 ) indicated that striplings are normally introduced to substance maltreatment through ‘gateway ‘ drugs such as intoxicant and coffin nails. Furthermore, late emerging surveies suggest that male striplings use and abuse drugs more than their female opposite numbers ( Igwe, Ojinnaka, Ejiofor, Emechebe, & A ; Ibe, 2009 ) . Though, female striplings favor stimulations ( Chassin, Ritter, Trim, & A ; King, 2003 ) .In line with the above, substance maltreatment among striplings continues to be a important public wellness concern.

Irrespective of the recent national informations collected in the US which shows lessenings among eighth-graders, 13 % of the group were still reported as holding abused substances in the past 12months ( Johnston, O’Malley, Bachman & A ; Schulenberg, 2008 ) . The writers stated that 5.5 % had reported holding been intoxicated. The theoretical underpinning of adolescent substance maltreatment lies within the context of societal cognitive theory ( Bandura, 1986 ) and changing grades of influence as espoused by Bronfenbrenner ( 1979 ) . These theories jointly maintain that parents, sibling, school, and equals account for an striplings overall motor to mistreat drugs.

Harmonizing to Baron and Kalsher ( 2008 ) striplings involved in substance maltreatment do so because of the demand to conform to others around which underscores the demand for belongingness. The bookmans stated that striplings learn to utilize consciousness changing drugs because they are by and large in trend. In the position of Dennis and Scott ( 2007 ) an person who developed substance maltreatment jobs initiated the usage during adolescence. To buttress their statement, the bookmans contended that 85 % of about 600 young persons come ining outpatient intervention for marihuana maltreatment or dependance in the hemp young person intervention survey started substance maltreatment before the age of 15 ( Dennis et al. , 2004 ) .

The impression of integral household and engagement in spiritual activities has been reported by so many bookmans as a protective factor in the etiology of substance maltreatment ( Wagner et al. , 2010 ; Demuth & A ; Brown, 2004 ; Grunbaum, Kann, Kinchen, Williams, & A ; Ross, 2002 ; National Center on Addiction and Substance Abuse, 2003 ; Chu, 2007 ; Mack, Leiber, Featherstone & A ; Monserud, 2007. In the position of Brook, Whiteman, Finch and Cohen ( 1998 ) many factors are basically interrelated, and have cumulative effects on the flights of drug maltreatment and delinquency.

Rohde, Lewinsohn, and Seeley ( 1996 ) stated that substance maltreatment heralded future depression in female striplings. With available grounds bespeaking that substance usage and behavior jobs before the age of 15 was among the strongest forecasters of chronic offending, depression, school failure, unemployment, relational jobs with equals and household members through adolescence into maturity ( McGue & A ; Iacono, 2005 ) .

1.2 Statement of job

The effects of substance maltreatment among striplings in Nigeria scope from a diminution in academic public presentation, hooky, stealing, contending, chancing and dependence ( Okoza et al. , 2009 ) . Epidemiologic ratings in the state indicate that substance maltreatment is widespread and is one of the most alarming health-related jobs among striplings ( Igwe, Ojinnaka, Ejiofor, Emechebe, & A ; Ibe, 2009 ) . Evidence suggests that the beginning of substance maltreatment is multi-faceted crossing assorted spectrums which includes biological, personal and societal surroundings ( Igwe et al. , 2009 ) . Studies therefore suggest that substance usage among striplings in Nigeria usually occurs in schools, with current estimations confirming the incidence of the phenomenon as high among striplings ( Eneh & A ; Stanley, 2004 ) .

Available informations from school studies in Nigeria farther reveal lifting prevalence and diminishing age of oncoming in reported instances of substance maltreatment ( Igwe et al. , 2009 ; Okoza, et al. , 2009 ) . The prevalence degree of the phenomenon was put at 33.7 % , with Alcohol noted as the most normally abused substance ( 31.6 % ) , while hemp was reported as the least ( 4.1 % ) abused substance among Nigerian striplings ( Igwe et al. , 2009 ) . Surveies have besides revealed a rise in ingestion, early induction, increasing female engagement and a tendency in the way of multiple substance usage among striplings in Nigeria ( Igwe et al. , 2009 ) .

Oshodi, Aina and Onajole ( 2010 ) contend that the prevalence rates for life-time usage of substances varied from 3.8 % for Heroin and Cocaine to 85.7 % for psycho-stimulants. In the state, current use of substances the bookmans revealed varied from 2 % to 56.5 % . For ” gateway drugs, life-time prevalence was estimated to run from 9.2 % to 5.2 % for intoxicant and baccy severally. However, the life clip use of hemp was put at 4.4 % . With respect to gender, prevalence estimations for males were by and large higher than for their female opposite numbers, except for antibiotics, anodynes heroin and cocaine.

However, diverse grounds have been adduced by bookmans as to why adolescent maltreatment drugs, some of which include alleviation from emphasis, to handle unwellness, and to remain awake at dark to analyze ( Oshodi, Aina & A ; Onajole, 2010 ) , still there is a few documented surveies on the inclination of striplings substance maltreatment. This survey therefore intends to make full an bing spread found in the literatures by researching outstanding issues or variables comparatively under studied by bookmans in adolescent substance maltreatment. Some of these issues include the function of self-esteem, emphasis, equal relationship and depression in the anticipation of substance maltreatment among striplings.

Despite the being of limited literature peculiarly on the function of self-pride, nevertheless findings remain contradictory. This survey hence seeks to clear up some of the built-in constructs and misconceptions on adolescent substance maltreatment by placing the alone forecaster of the concept substance maltreatment from the host of variables posited for the current survey. Although bookmans agree that the beginnings of substance maltreatment are multi-faceted in nature, nevertheless, few bookmans have examined this multi-faceted nature of substance maltreatment in their surveies peculiarly within the Nigerian context. This survey therefore fills a spread in the literatures by acknowledging the influence of assorted concepts from different spheres in the prognosis of substance maltreatment. This survey is therefore multi-dimensional in capturing aspects from self-esteem, stress, peer-relationship, depression and household background.

In surveies with young person in intervention for substance maltreatment, striplings tended to get worse more frequently in state of affairss of direct or indirect societal force per unit area ( 66 % ) compared with grownups ( Ramo & A ; Brown, 2008 ) . In general, the few work on female striplings leaves open the inquiry of temporal sequencing with certain substance maltreatment comorbid factors. Earlier surveies have provided reasonably consistent findings in footings of the protective function of parental monitoring ( Macauly et al. , 2005 ; Parker & A ; Benson, 2004 ) and support ( Olvera, Poston, & A ; Rodriguez, 2006 ; Simantov et al. , 2006 ) .

However, most old surveies have non focused on Africans and peculiarly Nigerians. It is clear that among both grownups and striplings, multiple personal and environmental factors influence adolescent substance usage ( Brown & A ; Ramo, 2006 ; Witkiewitz & A ; Marlatt, 2004 ) . The underlining subject of the current survey therefore is to detect how striplings can be protected from substance maltreatment, therefore the usage of concepts like integral household and spiritual activity as go-betweens in the survey on the relationship between self-pride, emphasis, equal relationship, depression, household background and substance maltreatment among striplings in Somolu, Lagos, Nigeria.

1.2.1 Research inquiry

In line with the statement of job, the undermentioned research inquiries were raised to make full the bing spreads in the research literatures reviewed on the relationship between self-pride, emphasis, equal relationship, depression, household background and substance maltreatment among striplings in Somolu, Lagos, Nigeria

In position of the identified spreads, the survey will react to the undermentioned research question:

1. What is the background information of striplings in Somolu, Lagos, Nigeria?
2. What is the age and gender difference in substance maltreatment among striplings in Somolu, Lagos, Nigeria?
3. Is there any relationship between self-pride, emphasis, equal relationship, depression, integral household, spiritual activity and substance maltreatment among striplings in Somolu, Lagos, Nigeria?
4. What is/are the forecaster ( s ) of substance maltreatment ( self-esteem, emphasis, equal relationship, depression ) ?
5. Is there any interceding consequence of integral household and spiritual activity on the relationship between self-pride, emphasis, equal relationship, depression and substance maltreatment among striplings in Somolu, Lagos, Nigeria?

1.2.2 Research aim

The research aim of the current survey will be captured chiefly from two aspects or dimensions. These dimensions include the chief and the specific aim of the survey as captured below.

1.2.2.1 General aim

The ultimate end of this research is to find the relationship between self-pride, emphasis, equal relationship, depression, household background and substance maltreatment among striplings in Somolu, Lagos, Nigeria.

1.2.2.2 Specific aim

The chief aim is supported by the undermentioned exact aims:

1. To depict the background information of striplings in Somolu, Lagos, Nigeria.
2. To find the age and gender difference in substance maltreatment among striplings in Somolu, Lagos, Nigeria.
3. To find the relationship between self-pride, emphasis, equal relationship, depression, integral household, spiritual activity and substance maltreatment among striplings in Somolu, Lagos, Nigeria.
4. To find the alone forecaster ( s ) of substance maltreatment from self-esteem, emphasis, equal relationship and depression among striplings in Somolu, Lagos, Nigeria.
5. To find the interceding consequence of integral household and spiritual activity on the relationship between self-pride, emphasis, equal relationship, depression and substance maltreatment among striplings in Somolu, Lagos, Nigeria?

1.2.3 Research Hypotheses

In response to the specific aims of the current survey, the undermentioned nothing hypotheses were formulated:

1. Ho1: There is no important age difference in substance maltreatment among striplings in Somolu, Lagos, Nigeria.
2. Ho2: There is no important gender difference in substance maltreatment among striplings in Somolu, Lagos, Nigeria.
3. Ho3: There is no important relationship between self-pride and substance maltreatment among striplings in Somolu, Lagos, Nigeria.
4. Ho4: There is no important relationship between emphasis and substance maltreatment among striplings in Somolu, Lagos, Nigeria.
5. Ho5: There is no important relationship between equal relationship and substance maltreatment among striplings in Somolu, Lagos, Nigeria.
6. Ho6: There is no important relationship between depression and substance maltreatment among striplings in Somolu, Lagos, Nigeria.
7. Ho7: There is no important relationship between integral household and substance maltreatment among striplings in Somolu, Lagos, Nigeria.
8. Ho8: There is no important relationship between spiritual activity and substance maltreatment among striplings in Somolu, Lagos, Nigeria.
9. Ho9: There is no important alone forecaster of substance maltreatment from self-esteem, emphasis, equal relationship and depression among striplings in Somolu, Lagos, Nigeria.
10. Ho10: There is no important interceding consequence of integral household on the relationship between self-pride and substance maltreatment among striplings in Somolu, Lagos, Nigeria.
11. Ho11: There is no important interceding consequence of integral household on the relationship between emphasis and substance maltreatment among striplings in Somolu, Lagos, Nigeria.
12. Ho12: There is no important interceding consequence of integral household on the relationship between equal relationship and substance maltreatment among striplings in Somolu, Lagos, Nigeria.
13. Ho13: There is no important interceding consequence of integral household on the relationship between depression and substance maltreatment among striplings in Somolu, Lagos, Nigeria.
14. Ho14: There is no important interceding consequence of spiritual activity on the relationship between self-pride and substance maltreatment among striplings in Somolu, Lagos, Nigeria.
15. Ho15: There is no important interceding consequence of spiritual activity on the relationship between emphasis and substance maltreatment among striplings in Somolu, Lagos, Nigeria.
16. Ho16: There is no important interceding consequence of spiritual activity on the relationship between equal relationship and substance maltreatment among striplings in Somolu, Lagos, Nigeria.
17. Ho17: There is no important interceding consequence of spiritual activity on the relationship between depression and substance maltreatment among striplings in Somolu, Lagos, Nigeria.

Theoretical/conceptual model

Although different theoretical predications possibly used in explicating substance maltreatment among striplings, nevertheless the present survey will use societal cognitive theory in explicating the diverse relationship that exist among the variables of the survey. On the other manus, ecological systems theory will be used to explicate stress one of the variables used for the survey. Based on the two theoretical predications, the conceptual model for the survey was built.

Theoretical model

The theoretical model of the survey will be guided by the societal cognitive theory as captured by Albert Bandura. The theory was chosen based on its rightness for the current rational exercising and based on its used by most of the research workers whose surveies were reviewed for the present academic job. The implicit in dogmas of societal cognitive theory are herewith discussed below.

Social Cognitive Theory

The term societal knowledge implies a broader aggregation of mental activities than societal acquisition ( Thomas, 2005 ) . The cosmopolitan civilization and constellation of societies, communities, groups, and other contexts provide larning chances that determine what is accepted and condemned by striplings. The presence and reaction of people ( for case in refering to societal countenances ) and the continuance of other stimulations attach dissimilar reinforcing or wash uping punishment to persons ‘ behaviour. Social construction can be conceptualized as an apprehension of agendas of beef uping exigency and other societal behavioural variables ( Bandura 1969 ) .

Behavior Factors

Cognitive, affective, biological events

Socio Cognitive Theory of Reciprocal Determinism. Adapted from Bandura ( 1969 )

The household, equals, schools, churches, and other gathering offer instant backgrounds that encourage or deter adolescent substance usage. Social cognitive theoretician propose that most striplings larning comes from active imitation or mold of what they see and hears theoretical accounts in the society do. Bandura used the word mold along with such footings as experimental acquisition and vicarious acquisition ( Bandura 1969 ) . Adolescents learn from experience by hive awaying in their memory the consequences of their incidental observation for usage at some ulterior appropriate clip. Therefore, they combine different sunglassess of behaviours to organize new behavioural paradigms.

Ecological Systems Theory

Ecological systems theory as propounded by Bronfenbrenner ( 1979 ) and other aligned bookmans focus on five primary universes of the stripling ( household, equals, school, society and the planetary environment ) 1 ) micro-systems, or the immediate societal contexts that straight influence striplings ( household, school, and equals ; Pantin, Schwartz, Sullivan, Coatsworth, & A ; Szapocznik, 2003 ) ; 2 ) meso-systems, or the connexions between the striplings ‘ universes ( parental engagement in school ) ; 3 ) exo-systems, or the fortunes in a parent ‘s life that indirectly influences striplings ( work emphasis, societal support ) ; 4 ) macro-systems, or the cultural or social ideals that describes a society or civilization ( norms, imposts, belief ) . It influences what, how, when and where striplings carry out their relationship ( Bronfenbrenner, 2005 ) 5 ) chrono-system or the history of relationships in an striplings household ( Swick & A ; Williams, 2006 ) .

Harmonizing to Pantin, Schwartz, Sullivan, Coatsworth and Szapocznik ( 2003 ) and in line with Bronfenbrenner ( 1979 ) these contexts besides can interact with one another. For illustration, a supportive household or school environment can buffer the harmful effects of vicinity disorganisation, poorness, and force on delinquent striplings ( Cicchetti & A ; Aber, 1998 ) . In the position of Pantin et Al. ( 2003 ) , the household context has the most influence on the stripling. In line with this propositions and sing the cardinal function of the household in civilizations ( Miranda, Bilot, Peluso, Berman, & A ; Van Meek, 2006 ) , household features may be an particularly of import influence on substance usage among Nigerian striplings.

Basically, ecological-contextual intercession paradigms have been suggested ( Biglan, 1995 ) , chiefly for early intercession attempts, given the significance of societal contextual factors in finding developmental paths ( Cohen & A ; Siegel, 1991 ) . Basically, family-based multiple-systems-oriented intercessions are compellingly advocated and extensively investigated ( Drug Strategies, 2005 ) .

Conceptual model

From the research inquiries, aims and posited hypothesis, the conceptual model for the survey is as shown below:

Background Independent Mediating Dependent

Conceptual model of the survey on ” Relationship between self-pride, emphasis, equal relationship, depression, household background and substance maltreatment among striplings in Somolu, Lagos, Nigeria ”

Definition of footings

In this subdivision, effort will be made to operationally specify the constructs to be used in the survey, particularly the dependant, independent and interceding variables of the survey. The definition is to guarantee unvarying specific mentions across the assorted spectrums of the thesis.

Substance maltreatment

Operational definition

The respondents entire mark on the 20item Drug Abuse Screening Test ( DAST: Skinner, 1982 ) will be used to stand for substance maltreatment in the survey. High tonss will bespeak high substance maltreatment.

Self-esteem

Operational definition

The respondents score on the 10item Rosenberg self-esteem graduated table ( Rosenberg, 1965 ) will be used to stand for self-pride in the survey. High mark will bespeak high self-prides, while low mark will bespeak otherwise.

Peer-relationship

Operational definition

The respondent ‘s entire mark on the 20item equal relationship questionnaire ( Rigley & A ; Slee, 1993 ) will be used to stand for equal dealingss in the survey. High mark indicate high equal relationship.

Stress

Operational definition

The respondents score on the 14item perceived emphasis graduated table ( Cohen, 1983 ) will be used to stand for emphasis in the survey. High tonss will bespeak high emphasis in the survey.

Depression

Operational definition

The respondents score on the 27item Children ‘s Depression Inventory ( Kovacs, 1985 ) will be used to stand for depressive symptomatology among striplings in the survey. High tonss will bespeak high depression in the survey.

Integral household

Operational definition

The term integral household is used to stand for striplings populating with both parents ( male parent and female parent ) .

Religious activity

• Operational definition
• The term spiritual activity is used to mention to the degree of adolescent engagement in spiritual activity.
• Adolescent
• Operational definition
• The term stripling as will be used in the survey implies anybody between ages of 10 to 19years.

1.5 Significance of the survey

• The survey can supply the necessary consciousness on the dangers of stripling substance usage and by so making trigger treatments on the topic among policy shapers in the state.
• The survey will assist edify households and communities on the hazard and protective of adolescent substance maltreatment.
• The survey will underline the demand for early intercession for striplings at hazard for substance maltreatment given its manifest and latent effects.
• The survey will besides make the consciousness of “ gateway ” drugs such as coffin nail normally abused by striplings.

1.6 Restrictions of the survey

• Despite the identified importance of the survey as captured in the significance, there are several likely restrictions that warrant consideration. These awaited restraints include the followers:
• The sample of the survey will merely be selected from secondary schools in Somolu local authorities country of Lagos, Nigeria.
• The information for the survey will merely be collected at one clip point ( cross-sectional ) .
• The full instrument to be used in the survey will be based on self-report.
• Given the sensitiveness of the capable affair, pupils may non give the needed information.

Chapter II

Literature Review

The chapter will reexamine literatures on self-pride, emphasis, equal relationship, depression, integral household, spiritual activity and substance maltreatment. The reappraisal will therefore uncover a causal relationship between the independent, interceding and dependent variables of the survey. In the position of O ‘ Malley, Johnson, Bachman and Schulenberg ( 2000 ) substance maltreatment typically emerges during adolescence. Although there are some gender differences in adolescent substance usage ( Igwe, Ojinnaka, Ejiofor, Emechebe, & A ; Ibe, 2009 ) , available grounds indicate that male striplings use and abuse drugs more than their female opposite numbers. However, female striplings favor stimulations ( Chassin, Ritter, Trim, & A ; King, 2003 ) . Epidemiologic surveies conducted in the present decennary suggest that substance maltreatment symptoms addition steadily across adolescence peculiarly among female striplings ( Johnson, Cohen, Kotler, Kasen, & A ; Brook, 2002 ) .

Earlier literatures based on the comparing of young persons populating in integral versus disrupted households have suggested that life with both parents may hold a protective consequence on stripling substance usage ( Grunbaum, Kann, Kinchen, Williams, & A ; Ross, 2002 ; National Center on Addiction and Substance Abuse, 2003 ) . More so, late emerging literatures contend that the issue of adolescent substance usage was more complex than originally believed. The impact legion bookmans have argued varies with gender, figure of household passages, quality of relationship with parent, alterations in socioeconomic resources, possible break of equal relationships, and other factors ( Hair, Park, Ling, & A ; Moore, 2009 ; Krohn, Hall, & A ; Lizotte, 2009 ) .

Shelef, Diamond, Diamond and Myers ( 2009 ) examined whether having substance usage upset intervention had a differential impact on coffin nail smoke behaviours of mild ( 10 coffin nails per twenty-four hours ) , moderate ( 10-19 ) , and heavy ( 20 ) tobacco users ( smoked on 60 yearss in the past 90 ) utilizing 378 striplings from the Cannabis Youth Treatment survey. Findingss of the survey indicated that mild tobacco users decreased yearss of smoking during intervention and followup, whereas centrist and heavy tobacco users demonstrated a little lessening over intervention, and no alteration over follow-up. More so, the writers noted little lessening among heavy tobacco users during coffin nails per twenty-four hours intervention stage. The findings of the survey affirmed the impression that although smoke may diminish for mild tobacco users, nevertheless moderate and heavy tobacco users require more attending.

In add-on, research besides affirm that depressive symptoms and substance usage portion relationships with of import hazard and protective factors, such as parental abnormal psychology, rearing jobs, kid exposure to force, school jobs, sexual activity and love relationships ( Harrison & A ; Sidebottom, 2009 ) . Studies every bit indicate that the developmental path of substance maltreatment symptoms rely chiefly on the age of oncoming, with initial oncoming of substance maltreatment before the age of 15 associated with increasing maltreatment for misss ( Chassin, Pitts, & A ; Prost, 2002 ; Nagin & A ; Tremblay, 2001 ) . Besides, well-known organic structure of research, both longitudinal and cross-sectional has demonstrated that adolescent substance maltreatment and hardship were associated with hapless mental wellness results among grownups ( Grella, Stein, & A ; Greenwell, 2005 ) .

Harmonizing to Ramo and Brown ( 2008 ) a major focal point in research analyzing the procedure of dependence impairment has been the word picture of diminution “ determiners, ” or contextual characteristics of state of affairss in grownups and striplings after been in intervention for substance maltreatment related jobs. Ramo and Brown ( 2008 ) examined adolescent and big substance maltreatment backsliding utilizing person-centered research attack by Karl Rogers ( 1957 ) in bring outing of import developmental differences in the state of affairss that make striplings and grownups most susceptible to get worse after substance maltreatment intervention. Findingss of the bookmans revealed that both striplings and grownups demonstrated two category agreements of backsliding precursors. Adults were labeled based on societal and urge state of affairss, negative and urges state of affairss. However, adolescent categories were labeled as societal and positive state of affairss and complex state of affairss.

Abundant grounds suggests that engagement in spiritual activities and integral households serve to protect or cut down the hazard of substance usage. Adolescents who attend church or mosque at least one time monthly reportedly may prosecute in smoke or imbibing but are significantly less likely to utilize marihuana and cocaine, compared with those who infrequently or ne’er attend church ( Chu, 2007 ) . Mack, Leiber, Featherstone and Monserud ( 2007 ) noted that individual parents may be less able to supply consistent supervising and monitoring for their kids, so striplings from individual parent families have more chances to experiment with substance usage and other delinquent behaviours in comparing with striplings from two-parent families. Other surveies such as Barrett and Turner ( 2006 ) confirmed the mediating function of the usage and blessing of substances by equals and exposure to emphasize.

In the position of Amato and Fowler ( 2002 ) , the place of household kineticss has been examined as both a correlative and a forecaster of adolescent substance usage. Family processes harmonizing to the bookmans act as a signifier of informal societal control that can diminish the chance of delinquent stripling behaviours by plumping chances to take part in aberrant behaviours, while supplying utility pro-social activities and promoting positive development. Available grounds from literatures indicates that female substance maltreatment enlargement is interwoven with antisocial, depressive, and eating upsets symptomatology ( Angold et al. , 1999 ) . Studies therefore denote that substance maltreatment symptoms typically result in the oncoming of other perturbations, particularly antisocial symptoms ( Brook, Cohen, & A ; Brook, 1998 ) . Rohde, Lewinsohn, and Seeley ( 1996 ) discovered that intoxicant maltreatment heralded future depression in female striplings.

Research grounds from both earlier and later surveies suggest that striplings who live in an agreement other than with their two biological parents study more substance usage than those who live with both parents ( Wagner et al. , 2010 ; Demuth & A ; Brown, 2004 ) . Harmonizing to Chassin et Al. ( 2005 ) , populating with other people was associated with coffin nail smoke in a sample of largely white, 10 to 17-year-olds, even after commanding for features of rearing manner. Barrett and Turner ( 2006 ) stated that others agreements like life with other non biological parents was associated with elevated DSM-IV substance maltreatment and dependance symptoms, intensified coffin nail smoke ( Miller & A ; Volk, 2002 ) and smoking beginning ( Edelen, Tucker, & A ; Ellickson, 2007 ) . However, the bookmans revealed that the presence of an grownup or older sibling who smoked counteracted the protective consequence of the atomic household.

Surveies have repeatedly besides found an opposite relationship between parental monitoring and adolescent substance usage ( Macauly, Griffin, Gronewold, Williams, & A ; Botvin, 2005 ; Parker & A ; Benson, 2004 ) . For case, in an earlier longitudinal survey of urban, Afro-american striplings Chilcoat and Anthony ( 1996 ) found lower quartile of parental monitoring in in-between childhood ( 8 to 10 old ages old ) was about three times more likely to originate the usage of cocaine, marihuana, and inhalants four old ages subsequently.

In a survey conducted by Parker and Benson ( 2004 ) lower degrees of parental support ( perceived parental trust, apprehension, equity, and pride ) were associated with increased stripling usage of intoxicant, cocaine and marihuana in a big, ethnically diverse national study. In discrepancy to the above survey, a nationally representative study conducted by Simantov, Schoen and Klein ( 2006 ) revealed that striplings who reported high parental support and frequent communicating were about half as likely to smoke and imbibe as their opposite numbers who reported infrequent communicating and did non place their parents as foundations of encouragement.

Chapter III

Methodology

The survey will consist junior secondary category three and senior secondary categories one to three pupils from selected schools in Somolu local authorities country of Lagos, Nigeria. Given that substance maltreatment among striplings peculiarly in Nigeria starts from schools. Therefore the school population will be the best topographic point for early sensing and bar of substance maltreatment among striplings ( Okoza et al. , 2009 ; Igwe, Ojinnaka, Ejiofor, Emechebe, & A ; Ibe, 2009 ) .

The sample pupils will be drawn from three public schools in Somolu city. The city is a assorted urban-suburban community that is socioeconomically representative of the province population. The sample of pupils was chosen because they provide the most accessible theoretical account of striplings who may hold come in contact with assorted substances while in school. Approval for the survey will be obtained from the Lagos State Ministry of Education and from the principals of take parting schools.

3.1.1 Location

The survey will be conducted in Somolu Local Government country of Lagos. Somolu is bounded by 3rd Mainland Bridge in the East, Bariga in the South, Atunrase Estate in the North and Ikorodu in the West. The country covers about 11.6km2 of land, with an estimated population of 402, 673 people ( Census, 2006 ) . Somolu is a strategic location peculiarly in the widely distributed metropolis of Lagos. It easy links topographic points like Lagos Island, Obalende, Surulere and of class Akoka where the University of Lagos is located. The country harbors some of the major markets in Lagos and the oldest secondary school in Nigeria. As a consequence, people from different cultural groups reside in the country.

3.1.2 Research Design

The survey will chiefly be a correlativity survey, aimed at finding the strength and way of relationship between the variables of the survey. It is besides aimed at turn toing concerns such as the extent of substance maltreatment among school traveling striplings in Somolu, Lagos, Nigeria.

3.1.3 Research Inclusion Criteria

To be eligible for engagement in the survey, striplings have to be between the ages of 10 and 19 old ages, in line with WHO specified categorization of adolescent age scope. The age scope will farther be divided into early ( 10-13 old ages ) , mid ( 14-16years ) and late ( 17-19 old ages ) in consonant rhyme with earlier surveies conducted by Igwe et Al. ( 2009 ) on the socio-demographic correlatives of psychotropic substance maltreatment among secondary school pupils in Enugu State, Nigeria.

3.1.4 Research Exclusion Criteria

Adolescent from selected schools in Somolu local authorities below the age of 10years and above the age of 19years as at last birthday will be excluded from the survey.

Sampling

Cluster trying technique will be used to choose a sum of 370 striplings. The trying method was chosen for the survey due to the homogeneousness of the sample. More so, the technique was preferred due to the trouble associated with obtaining a sampling frame. The sample of the survey will consist English-speaking adolescent pupils from Somolu, Lagos, Nigeria.

Owing to the indispensable nature of sample size in the statistical design of any research, it hence became of import to hold equal sample size. This is of import because equal sample size ensured dependable consequences. The reappraisal of literatures suggested that some statistical techniques were effectual in the finding of sample size. These consist of consequence size index, important standard and statistical illation power.

Since these parametric quantities are interrelated, if the research worker knows one, it was possible to find the others. There are besides some well-known regulations for finding each parametric quantity, for case when finding the power for a survey, it can be set at.80 ( Cohen, 1988 ) . Another common regulation related to important degrees, harmonizing to Cohen ( 1997 ) was that in most instances a.05 value was acceptable. On the other manus, for two tailed trials, where the alternate hypothesis shows that Ma # Mb, we can conventionally utilize medium Es, assumed as equal to.5. For the intent of the current survey on relationship between self-pride, emphasis, equal relationship, depression, household background and substance maltreatment among striplings in Somolu, Lagos, Nigeria. Krejcie and Morgan ( 1972 ) methodological attack for the finding of sample size was adopted. The expression stated by the bookman is herewith captured below:

n = X2 *N*P* ( 1-P )

( ME2* ( N-1 ) + ( X2*P* ( 1-P )

Where:

n = sample size

X2 = Chi – square for the specified assurance degree at 1 grade of freedom

N = Population size

P = Population proportion ( .50 in the tabular array )

ME = Desired border of mistake ( expressed as a proportion ) ( See attached sample size tabular array ) .

Therefore, in line with above mentioned parametric quantities, the sample size for the current survey was determined. Basically, a one or two tailed survey depends on old findings, as there was no similar survey at the local degree, the hypothesis will be two-tailed.

Data Collection

The information for the survey will be obtained through self-report instruments administered to pupils in their schoolrooms by a trained research helper utilizing standardised protocols. After reading the instructions to the pupils, the research helper will go around the steps in the schoolroom and will reply any single inquiries about peculiar points. The study will be administered under confidential conditions, and a certification of confidentiality protecting the information will be obtained from the Lagos State Ministry of Education.

Students will be instructed non to compose their name on the study and will be assured that their replies will be purely confidential and would non be shown to their parents or instructors. Methodological research has shown that when participants are assured of their confidentiality, self-reports of substance maltreatment normally have good cogency ( Patrick et al. , 1994 ) . An approximative clip of disposal of instruments on participants of the survey will be determined during the pilot survey of the current research.

3.4 Instruments

The instrument brochure for the current survey will incorporate a combination of gages and single points designed to mensurate background features such as age, ethnicity, household composing ( integral, divorced or detached households ) , and other variables such as self-esteem, emphasis, equal relationship, depression and substance maltreatment.

Substance maltreatment

Substance maltreatment will be measured with the 20item Drug Abuse Screening Test ( DAST: Skinner, 1982 ) . The mark of DAST scopes from 0-20 with high mark stand foring substance maltreatment.

Stress

Stress will be measured by the Perceived Stress Scale ( PSS ) ( Cohen, 1983 ) , a 14 point self study questionnaire designed to mensurate the grade to which state of affairss in life are appraised as stressful. The tonss of the PSS are obtained by change by reversaling the tonss on positive points and so summing all the tonss of the 14 points. The PSS graduated table tonss range from 0 to 56 with high mark stand foring high societal emphasis.

Self Esteem

The 10-item Rosenberg Self-Esteem Scale ( Rosenberg, 1965 ) will be used to measure planetary self-pride, with higher tonss bespeaking positive dignity. Each point will be scored on a 4-point Likert graduated table runing from 1 = strongly agree to 4= strongly disagree.

Depression

Children Depression Inventory ( CDI: Kovacs, 1985 ) will be used to measure depressive symptomatology with higher tonss bespeaking high depressive symptomatology. The CDI consists of feelings and thoughts grouped into 27 points. The graduated table tonss range from 0 to 54.

Peer relationship

Peer relationship questionnaire ( PRQ: Rigby & A ; Slee, 1993 ) will be used to mensurate equal dealingss. The PRQ has three subscales: intimidation, equal victimization, and pro-social graduated table. The instrument is used for kids between the ages of 12 to 18year and consist of 20 points scored on a 4-point graduated table, runing from Never 1, Once in a piece 2, Pretty frequently 3, and Very frequently 4.

3.5 Data Analysiss

In analysing the information, the internal consistence of the graduated tables ( correlativity ) points will be measured utilizing Cronbach ‘s Alpha. The trial is undisputedly the most normally recognized step of dependability. Each subdivision of the questionnaire will be calculated individually. Before continuing into the reported tonss of the overall responses to each step, attending will foremost be given to the normalcy of the informations distribution.

The information will be inspected utilizing graphical shows such as histogram, root and foliage secret plan, box-plot and normal chance secret plan. The overall mean tonss for all the respondents along with standard divergence value will besides be calculated. In line with the aims of the survey, Independent sample t trial will be used to analyze difference, Pearson correlativity will be used to find the relationship between variables and hierarchal arrested development analyses used examine mediation.