Water

KNOWLEDGE ATTITUDES AND PRACTICES (KAP) END-LINE ASSESSMENT On Water, Sanitation and Hygiene LOLKUACH Village, IDPs of Akobo September-2012 DRC-Gambella WASH Team Conducted in the frame of an ECHO funded project “Improving access to short-term food security, safe drinking water, hygiene and basic household items in Ethiopia” Wanthowa Worda, Gambella, Ethiopia September 30, 2012 i TABLE OF CONTENTS 1 2 3 3. 1 INTRODUCTION SUMMARY OF FINDINGS METHODOLOGY Objectives of the Survey 1 2 3 3 4 4. 1 FINDINGS General Background Information 4 4 5 5. 1 5. 2 5. 3 WATER RELATED INFORMATION

Water Sources Water collection and storage Household Water Treatment 5 5 9 11 6 6. 1 6. 2 HEALTH AND HYGIENE Diseases Washing Hands and Good Hygienic Practices 12 12 15 7 7. 1 7. 2 SANITATION Defecation Waste and Waste Management 18 18 20 8 9 CONCLUSION RECOMMENDATIONS 23 24 25 10 REFERENCES i 1 Introduction The 2012 report states that as of end of 2010: Over 780 million people are still without access to improved sources of drinking water and 2. 5 billion lack improved sanitation. If current trends continue, these numbers will remain unacceptably high in 2015: 605 million people will be without an improved drinking water source and 2. billion people will lack access to improved sanitation facilities. An estimated 801,000 children younger than 5 years of age perish from diarrhea each year, mostly in developing countries. This amounts to 11% of the 7. 6 million deaths of children under the age of five and means that about 2,200 children are dying every day as a result of diarrheal diseases. Unsafe drinking water, inadequate availability of water for hygiene, and lack of access to sanitation together contribute to about 88% of deaths from diarrheal diseases (UNICEF, WHO, 2012: 2; Center of Disease Control and Prevention, 2012).

As to Andrea Naylor: although worldwide there have been thousands of projects to address water and sanitation issues as they relate to public health with continued improvements since the 1980’s, research has shown that due to lack of evaluation surveys on the effectiveness and success of these interventions, many are not sustainable . To this end, the essence of conducting end-line survey is very critical to gauge the effectiveness and success of the interventions of DRC-Gambella. The Gambella Region has an approximately population of 332,600 people, with 49,457 living in Akobo and Wantawo Woredas.

These populations are subjected to water shortage and floods. Moreover the population is prevalently pastoralist and follows seasonal migration patterns for cattle grazing and protection of livestock from drought and floods. The perennial attacks by the Murle tribe, coupled with intra-clan conflicts among the Nuer tribes of Ethiopia and South Sudan, aggravates a situation of chronic displacement, making populations of bordering areas, especially Akobo, susceptible of massive and prolonged internal displacements.

Conflicts, drought and floods are the key challenges to the populations in Akobo and in Wantawo. The consequent perennial movement makes the community vulnerable to food insecurity, disease and water shortage. It is in view of this that Danish Refugee Council seeks to address in the short term the basic needs of these populations by providing access to clean drinking water, and tools to improve hygiene and to build the capacity of the community to respond to these challenges. From the period of July 2011 to June 2012, DRC implemented a Water, Sanitation and Hygiene project, funded by ECHO, with the goal of rehabilitating 7 hand pumps (and subsequently chlorinating the water), distributing NFI kits, hygiene kits, and implementing hygiene promotions. DRC decided to conduct two in-depth KAP surveys (as a baseline and endline) to evaluate the impact brought by the implementation of the project in the targeted area.

The baseline survey was conducted in the month of May 2012 and the end line survey was conducted in the second week of September 2012. In the period between the two surveys, a number of activities covering water, sanitation and hygiene were implemented in the frame of the project. 2 Summary of Findings Project outputs and behaviour and knowledge change (as indicated by the pre and post implementation KAP surveys) indicate the following key findings: o o o o o o Seven hand pumps were rehabilitated/ disinfected Hygiene promotion targets were surpassed. planned: 5,490 beneficiaries; 10,950 reached) Hygiene kit distributions were surpassed (planned: 2,250 beneficiaries; 8,870 reached) NFI kit distributions were surpassed (planned 6,300 beneficiaries; 7,470 reached) The number of respondents who use hand pumps as source of water increased from 4% to 75% Knowledge and practice of feasible water purification practices such as boiling, filtration or adding tablet/sachet has been greatly improved Instance of diarrhoea has decreased from 60% to 24% of respondents stating that they had had diarrhea in during the 3 weeks prior to the survey Knowledge that rain water is a safe drinking water source has improved from 24% to 62% of respondents, however, the use of rain water remains limited.

Knowledge of the causes of unsafe drinking water (including germs, visible particles and bad taste) increased from 40% to 81%. The practice of open defecation has reduced from 100% to 15% of respondents. Hand washing at critical times has increased from 34% to 85% of respondents. 2 o o o o o o o Appropriate waste disposal mechanisms improved from 39. 2% in baseline to 75% of respondents.. Although there has been an improvement in the knowledge of respiratory and eye infection transmission/protection, there is still room for improvement 3 Methodology A cross sectional, qualitative study was conducted through house to house interviews, taking 150 respondents randomly as study subjects. The sample represents nearly 10% of the total targeted household 1 n Lolkuach village (1,500 household). The questionnaire (See Annex I) was employed to collect data on general background information, knowledge, attitude and practices of the IDPs of Lolkuach village. However the results can also be considered pertinent for the host communities if considering the cultural and environmental homogeneity. Verbal consent from the respondents was obtained after explaining the purpose of the study. Data was collected from 13 to 14 September 2012. The data from the questionnaires was entered into SPSS software (version 13) by the principal investigators for further analysis. Data reliability was assured using different techniques such as: ?

Properly designed questionnaires were prepared and pretested. ? Data collectors were hired locally and tested during the training on the contents of the questionnaire. Constant supervision was done by DRC WASH Team Leader, and problems encountered at the time of data collection were reported immediately and appropriate actions taken. 3. 1 Objectives of the Survey ? To identify gaps in knowledge regarding health and hygiene practices and existing practices leading to negative impact on health. ? ? To describe the socio demographic, cultural information of respondents and villages. To find out the information on incidence of communicable disease due to unhygienic practice. 1

It is estimated, on the base of IOM Akobo IDPs database, that the number of households currently living in Lolkuach is 1500 and average family size is 5. 3 ? To assess the effectiveness and impact of the DRC water, sanitation and hygiene promotion activities. 4 Findings 4. 1 General Background Information The beneficiaries of the programme, and KAP survey respondents are all part of the displaced NuerGajok population from Akobo Woreda now living in Wantawo. Among the KAP survey respondents, the majority (about 65 %) were female, whereas 35% were male. Females were particularly targeted for the KAP survey, as they were the primary recipients/participants in the DRC project, and are traditionally responsible for child care and household WASH issues.

This survey was conducted near the end of the rainy season, in Lolkuach IDP settlement. Respondents reported moving between the river banks temporary camps and dry land permanent villages according to seasonal variations. During the dry season, the majority of the respondents live in Dimbierow village (79%), and Nyawich village (17%), while only 4 % of the respondents indicated that they live in Lolkuach village throughout all the year. However there are frequent movements among the settlements throughout all the year. Most of the respondents (86. 2%) indicated that they arrived at Lolkuach between February and June 2009 following a recurrence of conflict with Lou Nuer in Akobo woreda.

Minority of the respondents arrived during the same period of 2008 (12. 8%) or 2010 (1 %). Most of the respondents therefore have been displaced since 2009. When respondents were asked if they plan to return to their villages of origin, a pronounced number (55%) indicated that they don’t have any plans to return due to security problems (expressed as ‘war’, ‘conflict’, ‘insecurity’). The remaining 45% of the respondents indicated that they plan to return back in the future if the security situation is restored and the construction of the road from Mathar to Akobo is finalized. In this regard, as it can be observed from the baseline survey, no significant difference noted in the end line survey.

However looking in detail at the positive answers (from the 45% of respondents), 21% expressed a plan to go back within six months and the remaining 34% indicated a time longer than six months. Moreover even the respondents who indicated that they have a plan to return back to 4 Kebele of origin also mentioned their fear about the security situation (expressed as ‘if peace come back’, ‘if cattle raiding ends’, if the construction of the road to Akobo is completed and similar). 5 Water Related Information 5. 1 Water Sources Before the project interventions, the baseline data indicated that almost 100% of the respondents were accessing unsafe drinking water from the river, which is contaminated from the presence of livestock and open defecation. At the end of the project implementation, the hand pump aintenance/rehabilitation/water chlorination, coupled with pure sachet distributions, bucket distributions, and hygiene promotions resulted in a significant positive change. As you can observe from the Figure 1, the majority of the respondents are now using water from newly maintained/rehabilitated hand pumps. Due to seasonal movement however, the proportion of respondents using hand pumps during the dry season reduces, as many of the beneficiaries move to areas without hand pumps. The following graph outlines both the shift in hand pump use (pre and post intervention), and also the relation of this use in terms of seasons. There are still not sufficient hand pumps in Lolkuach area to support the population however, which explains why 100% of the respondents are not using these protected sources.

Considering that the 7500 inhabitants of Lolkuach, Thore and Lolmokoney have only 7 hand-pumps (hand dug wells), this is insufficient as per SPHERE standards)2 , highlighting the need to construct new hand pumps. 2 Considering the maximum number of users for 1 hand pump should be 500, at least 15 hand pumps would be needed in Lolkuach 5 Seasonal Use of Protected Water Sources – Pre and Post Intervention 100 90 80 70 60 50 40 30 20 10 0 Dry Season Rainy Season % of Respondents Seasons Baseline Endline Figure 1: Shift in Use of Protected Water Sources (KAP baseline an d end-line) Seven hand pumps in Lolkuach and surrounding villages were disinfected and beneficiaries received pure sachet as well bucket and filter.

From the findings, the graph below states that it is only 27% of the respondents indicated that the main problems with their water source are water is dirty and it tastes bad. Whereas 40. 7% of the respondents also signified that the water source is far. Problems Related to Water Supply 100 90 80 70 60 50 40 30 20 10 0 Dirty Water Bad Taste Irregular FlowSource is Dried Distance to No problems Up Source % Respondents Baseline Endline Water Source Issues Figure 2: Main problems related to water supply. 6 Consequently 63% of the respondents consider the water they are using is safe for drinking, and 33% consider it is unsafe instead (Figure 3).

This represents a reduction in the proportion of respondents who stated that they were using unsafe water from 77% in the baseline to 33% in the end-line survey. Of these 33% of respondents who noted that they were drinking unsafe water, 8% of the respondents were using hand dug wells (Which were rehabilitated by DRC) as source of water for drinking. Figure 3: consideration of water safety Figure 4: reasons why 33% declared water is unsafe In relation to the safety of water, the reason why 33% of respondents declared that they are using unsafe water is mainly because the water contains germs, is not filtered and not cleaned. This shows that their understanding about the causes of unsafe water has improved since the baseline (Figure 4).

When it comes to use of rainwater as source, though improvement is registered, much needs to be done to bring about significant change. Considering the shortage of safe water sources in the area observed by DRC, and the abundant rain-fall in Gambella region3, reasons for not using the rainwater (which is almost distilled4) were assessed more closely. Although the number of respondents who believe that 3 The annual rain falls in Gambella region ranges between 800 and 1200mm, but about 85% of rains are concentrated between May-October (Woube, 1999). 4 In this regards, Dev Sehgal, indicated that rainwater harvesting is an easy method to collect drinking water, and the quality of the water is almost distilled.

First when the water touches the catchment surface it usually gets contaminated (Dev Sehgal, 2005). 7 rainwater is unsafe has reduced from 76% to 38% of respondents, more can be done to raise awareness on this water collection method. Of the 38% of respondents who would not collect rain water given the choice, the principal reasons were given as follows: Figure 5: Investigation about unused rain water When questioned on their knowledge of safe drinking water and water pollution causes, respondents were given the option of providing more than one answer. The number of respondents who indicated that drinking water shouldn’t have germs, visible particles and/or bad taste, increased from 40% at the baseline to 81. 3% at the end-line.

The respondents who indicated that the proximity of a latrine to water sources can cause water contamination increased from 7. 2% in the baseline to 15% in the end-line survey. In this regards, water quality and health council indicated that especially the proximity of latrine to water sources can cause Removing the first harvested water, so-called first flush, can prevent this. When the rain starts to fall the first water cleans the catchment surface and fills up the first flush diverter, by the time it is full a ball closes the opening and leads the water to the main tank. The downside of rainwater harvesting is that it requires double storage, as it is hard to purify water at the same speed as it rains (Gould, J. & Nissen-Petersen, E. , 2005). 8 contamination .

The majority of the respondents (85%) also indicated that garbage disposal or animals feces containers near a water source, or unprotected source can cause water contamination (Figure7). 5 Knowledge of Causes of Water Source Pollution 100 90 80 % Respondents 70 60 50 40 30 20 10 0 Defecation Nearby Garbage Nearby Dirty Container Causes of Pollution Figure 7: Knowledge of Water Source Pollutants Baseline Endline Although only a small proportion of respondents acknowledge that water can be contaminated through the ground from a latrine constructed too close to a water source, 95% of respondents are now aware that defecation near a water source is a pollutant, resulting in a change of behavior in which open defecation has reduced from 100% in the baseline to 15% in the end-line survey. 5. 2 Water collection and storage

From the Figure 8, it can be observed that nearly 50% of respondents less than 50 minutes to fetch water during dry seasons6, meaning that SPHERE standards for these respondents are met for watersource distance because of the rehabilitations of the hand pump in the vicinity of the village. Concerning rainy season, it can be observed that respondents spend more time getting water. As it is observed, respondents need to travel some distance to fetch water and during the dry season respondents also move to river banks. Hence, this can make the access to hand pump difficult. So besides constructing 5 The causes of water pollution vary and may be both natural and anthropogenic.

However, the most common causes of domestic water pollutions includes : garbage disposal and defecation near water sources, animals feces, sharing the same sources with animals, use of dirty or open water container can affect the safety of our water . Use (Water Quality and Health Councils, 2010; CAWST, 2009; Laurent, P. , 2005). 6 According to SPHERE key indicators, the maximum distance from any household to the nearest water point is 500 metres 9 new hand pumps, encouraging the community for rain water catchment strategy is very essential at household at household level. 70 60 50 40 30 20 10 0 0-50 50-100 Min 100-250 Min More than 250 Dry Season Rainy Season

Figure 8: Average time spent to collect water Given that water collection requires women and girls to walk distances to find water sources, there may be heightened protection issues for these family members, although protection was not assessed in the KAP. Question posed to respondents on what devices that they are using to store and collect water indicated that 55% of the respondents are using plastic jerry cans to collect water and 34% of the respondents use plastic bucket for water collection. For storing water, nearly 33% of the respondents use traditional clay pot and plastic jerry cans; the rest 36% of the respondents indicated plastic jerry cans or buckets with lid.

DRC distributed NFI (Contains 2 Jerry cans each 20 litters among others) and Hygiene kits (Contains 2 Buckets each 10 litters among other) to 302 and 283 households respectively living in Lolkuach areas. To this end, most of the respondents own more than one container. But still those who didn’t receive water storage and collection device also were among the respondents who took part in the survey, we can 10 observe that 70% of respondents meet the minimum SPHERE7 requirement for water collection container, and 74% meet the requirement8 for water storage. Whereas in the baseline, it was noted that only 50% of the respondents met the requirement for water storage and collection devices. 5. 3 Household Water Treatment

The knowledge of practical purification methods like boiling, filtration or adding tablet/sachet was assessed. As it can be observed from Figure 12, there is great leap in knowledge of the basic methods of household water treatment. For instance, use of purifying sachet/tablet increased from 8% at baseline to 85% at the end-line survey. The findings also suggested that the majority of the respondents (more than 75%) know the use of feasible practices like boiling, filtration or adding tablets/sachet for water treatments9. This figure was only 25% in the baseline survey. After the baseline survey, it is worth to note that DRC-Gambella has been distributing purifying sachet and providing demonstrations for those villages with no access to hand pumps. 7

According to SPHERE key indicator: Each household has at least two clean water collecting containers of 10-20 litres, plus enough clean water storage containers to ensure there is always water in the household. The amount of storage capacity required depends on the size of the household and the consistency of water availability e. g. approximately 4 litres per person would be appropriate for situations where there is a constant daily supply 8 Requirement for storage is calculated according to certain specificities, but considering the minimum of 4lt/person/day, for an average household of 5, should be at least 20 lt. 9 Different researchers suggested some feasible practices like boiling, filtration or adding Figuret/sachet and chlorination for water treatment (CAWST, 2009; Davis & Lambert, 2002). 11

Knowledge of Household Water Treatment 140 120 % Respondents 100 80 60 40 20 0 special container Boiling Use of sachet Cleaning Filtering container with cloth Covering sunlight Baseline Endline Figure 12: Knowledge of household water treatment methods 6 Health and Hygiene 6. 1 Diseases Respondents were asked about the diseases their family experienced during the three weeks before the interview. The number of respondents who caught diarrhea in the three weeks prior to the interview reduced from 60% in the baseline to 27. 3% in the end-line survey. Hence, you can see from the end-line survey that hygiene conditions and practices are improving.

When it comes to the causes of diarrhoea, more than 85% of the respondents referenced unsafe drinking water, children feces, germs/bacteria, open defecation, poor hygienic practices and flies as causes of diarrhea (Figure 16), indicating that the hygiene promotion has resulted in an increase in knowledge. 12 Figure 16: Knowledge about diarrhea transmission Interviewees were asked to indicate in a multiple choice question, which action to be taken to protect their families from the different diseases that they suffered from. The respondents who indicated that they can be protected from malaria by sleeping under mosquito net increased from 40% to 75%. Keeping the environment clean and good hygienic practices also attributed as a method of prevention of malaria by many respondents (Figure 14). 13 Knowldge of Malaria prevetion measure 120 100 Respondents 80 60 40 20 0 Keeping environment Clean Safe water Good hygienic practice Use mosquitonet Wash cloth Wash hand Baseline Endline Figure 14: knowledge of malaria prevention measures When it comes to skin diseases, most of the respondents indicated that good hygienic practice as way of prevention of skin diseases (Figure 15). 14 Figure 15: Knowledge of skin diseases prevention measur es Nearly 51. 2% of the respondents indicated that good personal hygiene, keeping the environment clean, use of safe water for drinking, washing hands, washing clothes and hanging them in the sun can protect their families from respiratory and eye problems.

The above results indicate that the knowledge of the people has improved with regards to respiratory illness and eye infection transmission and protection, however there is still room for improvement. 6. 2 Washing Hands and Good Hygienic Practices General question about hygiene and more specific ones about hand washing were posed. Keeping food away from flies, bathing regularly, keeping compounds clean, protecting food and washing hands are considered as good hygienic practices by the majority of the respondents in the end-line survey. This means that the figure increased from nearly 51% at the baseline to nearly 85% in the endline. 15 Figure 18: Knowledge about keeping good hygiene

Likewise, when respondents specifically asked if they wash their hands, 89% of the interviewees gave affirmative answer in the end-line Survey. People who wash hands reported to be doing it in order to eliminate bad smell and prevent diseases. Similarly more details of the hand washing practice can be seen from Figure 20, and it can be concluded that more than three fourth of the population who wash their hands, are doing it at the appropriate times. 16 Figure 20: Frequency of hand washing practice While the vast majority of the respondents (95%) stated they would like to bathe once a day, when it comes to practice, 29% of respondents expressed they have problems in taking bath regularly mainly because of lack of container and soap (Figure 21).

Hygiene practices were also considered to be a major issue by nearly 40. 6% of the respondents, these respondents indicated that poor practices are due to both a lack of access to hygiene items, and a poor attitude brought on by a lack of knowledge. So the majority of the respondents signified that the distributed hygiene kits solved some of their problems and they were adhering to good hygienic practices. 17 7 Sanitation 7. 1 Defecation Before the DRC intervention, the majority of the adults practiced open defecation. Because changing habits is not easy, the baseline assessment was designed to understand the risk practices that were most widespread and identify those that could be changed.

From the point of view of controlling diarrhoea, the priorities for hygiene behavioral change included hand washing at critical times and safe stool disposal. To this end, the efforts of the organization brought significant behavioral change. From the end-line survey it is noted that 85% of the respondents use traditional latrines, which is up from 0%. Similarly, when asked to indicate the best option for defecation, 85% indicated the latrine. On the other hand, privacy, water pollution, presence of bad smell and flies, as well as spread of disease was reported as the main problem related to open defecation practices (Figure 23). Respondents were also asked about post defecation cleansing habits and mostly indicated pieces of paper. Figure 23: Problems related to defecation practice 18

Considering the majority of respondents indicated that a latrine is the best option for defecation, and that the main issue with defecation is privacy, disease, water pollution, smell and environmental pollution, it was observed that the traditional latrine which is constructed by the participation of the communities has been welcomed and used by the community. In the baseline survey it was found out that inadequate sanitary conditions and poor hygiene practices played major roles in the increased burden of communicable disease within the village. Similarly, the baseline information stated that beneficiaries had problems with access to safe water and sanitation facilities. To this end, DCR Gambella set a strategy to solve the problems through community participation. DRC- Gambella inculcates the basic principles and approaches Sanitation) of into CLTS the (Community newly Lead Total PHAST designed Participatory hygiene and Sanitation Transformation) training. As both approaches opt for communities’ participations and empowerment and focus on igniting a change in sanitation and hygiene behaviour, a PHAST training manual that encompasses both PHAST methodology and catalysts for change in sanitation behaviour was prepared and distributed. After community based health promotions work, and community conversation establishments at each village, the accessibility to sanitation facilities and sanitation practices improved. 1446 households who completed hand washing points and traditional pit latrine (See the figure on the right side) were awarded NFI to recognize their efforts of behavioral changes.

Hand washing after stool contact and safe disposal of stool have been priorities in hygiene and sanitation promotion interventions in Wanthowa Woreda. By understanding that for the quickest and widest adoption of good hygienic practices it is often more cost-effective to rely on social ambitions rather than health arguments to encourage change, DRC linked hygiene promotion works with social and cultural values, norms as well as NFI distributions, such that all hygiene promotions were linked with cultural problems of Nuer society and social values. As a result good improvements in both hand 19 washing and safe stool disposal were registered. This can be confirmed by looking at the end line KAP survey results. 7. Waste and Waste Management The majority of disease measures are related to environmental conditions: appropriate shelter, clean water, good sanitation, and vector control, personal protection such as (insecticide-treated nets, personal hygiene and health promotion). Appropriate waste disposal mechanism is vital to avoid environmental pollution and breading place for vectors and pathogens. In this regards, the majority of the respondents (75%) indicated that they are now burning the household solid wastes on timely bases (Figure 24). The number of respondents who had been disposing solid wastes in open space and river significantly decreased after the interventions.

Figure 24: waste disposal practice 20 The problems concerning waste were indicated in flies, bad smell, breeding place for mosquitoes. Majority of the respondents understood that appropriate solid waste disposal plays a vital role in minimizing the breading of vectors and other pathogens (Figure 25). Figure 25: Problems related to waste disposal The majority of respondents indicated that the practice used to dispose household waste is burning. Improvement in waste disposal and keep the villages clean is observed by DRC field staffs. Similarly the views of the majority of the respondents on the attributes of clean and health village is improved.

It is noted that availability of safe water, cleanness of the village and availability of latrine considered by more than three fourth of the respondents as the attributes of clean and health village in the end-line survey. But those we stated the same were nearly 50% in the baseline survey. 21 Similarly, the benefits of keeping a village were mainly identified as decrease of diseases occurrence, improved beauty of village, minimized presence of mosquitoes and flies by more than three fourth of the respondents in the end-line where as this nearly 53% in the baseline. From end-line survey, it can be inferred that majority of respondents indicated that important public health factors such as availability of safe water and atrines, absence of stagnant water and mosquitoes among the attributes of an healthy village. They also noted that this has great impact in reduction of infection disease prevalence. Hence, it can be concluded that the understanding of the majority of the respondents on disease transmission, transmission routes and its preventions tremendously improved after the interventions. 22 8 Conclusion Diarrhoea causes dehydration and kills approximately 2. 2 million people, mostly children, every year. Children are more likely than adults to die from diarrhea because they become dehydrated more quickly. In the past 10 years, diarrhea has killed more children than all of the people lost to armed conflict since World War II.

Its occurrence is closely related to the opportunities that poor people (especially poor mothers) have to improve domestic hygiene10. Diarrhoea does not only cause disease and early death in children, but also affects children’s nutritional status, stunting children’s physical and intellectual growth over time. Skin and eye infections are especially common in arid areas. Both diarrhoea and other infectious diseases have health as well as socio-economic consequences. Washing more often can greatly reduce their spread11 . Similarly, the training manual of Amhara region indicated that improved hygiene, particularly hand washing at critical times can reduce diarrhea by one third and reduce malnutrition12. Soiled hands are an important source of transmitting diarrhoeas.

Recent research also suggests that hand washing is an important preventive measure in the incidence of acute respiratory infections, one of the top killer of children under five. 13 This KAP survey was conducted in order to compare its results with the results of the baseline survey, to identify whether the hygiene promotion activities conducted in the frame of the ECHO funded project had been effective. The baseline and end-line survey results revealed that positive results have been achieved in the overall hygiene situation. In the baseline survey the situation was poor i. e. lack of safe water, poor sanitation facilities, poor hygiene practice etc. At the end of the project, an improvement was noted in the overall hygiene and sanitation behaviour.

Though improvements were noticed after the implementation of project, it should not be forgotten that it takes time to consolidate behaviour changes, so more follow up is necessary for further improvement. 10 11 12 (Curtis et al. , 2000). Brian Appleton and Christine van Wijk (IRC), 2003. Amhara Regional State Health Bureau, 2011; Isabel Carter, 2005 13 See for instance the study of Ryan et al. published in 2001 23 9 RECOMMENDATIONS Although the WASH project can been seen as a success, the team noted some recommendations for future interventions. ? ? Construct 15 shell wells in Lolkuach village so that inhabitants meet SPHERE standards Assess whether it is possible to dig wells in the locations where people move to during the dry season ?

Introduce rain water harvesting techniques, which are easy sources of potable water and would reduce the distance travelled to access water, thus improving the protection status of the women and girls that are responsible for this task. ? ? Follow up on well water quality in rehabilitated wells Although respondents recognized that animal feces can contaminate water, only 15% in the end-line noted that the proximity of a latrine to a water source can contaminate drinking water. This could be stressed and improved in future hygiene promotion activities. 24 10 References 1. Amhara Regional State Health Bureau (2011). Training Manual on Hygiene and Sanitation Promotion and Community Mobilization for Volunteer Community Health Promoters (VCHP)/ Draft for Review. Online Available at: http://pdf. usaid. gov/pdf_docs/PNADP828. pdf 2. Andrea Naylor.

Development and Implementation of Sanitation Survey Using a Knowledge Attitudes Practices (KAP) Model. University of South Florida (Tampa): CGN6933 “Sustainable Development Engineering: Water, Sanitation, Indoor Air, Health” and PHC6301 “Water Pollution and Treatment”. 3. Brian Appleton and Christine van Wijk (IRC) (2003). Hygiene Promotion Thematic Overview Paper. IRC International Water and Sanitation Centre 4. Boot, Marieke T. and Cairncross, Sandy (1993). Actions speak: The study of hygiene behaviour in water and sanitation project. The Hague: IRC International Water and Sanitation Centre. 5. CAWST (Centre for Affordable Water and Sanitation Technology) (2009) Household water treatment and safe storage factsheet: natural coagulants.

Online Available at: http://cawst. org/en/resources/pubs/file/38-hwts-fact-sheets-academic-english 6. Davis, J. and Lambert, R (2002) Engineering in emergencies – A practical guide for relief, workers 2nd edition, Rugby: Practical actions publishing 7. Dev Sehgal, J. (2005) A guide to rainwater harvesting in Malaysia. Online Available at: http://www. wasrag. org/downloads/technology/A%20Guide%20to%20Rainwater%20Ha rvesting%20in%20Malaysia. pdf 8. Esrey, S. A. (1994). Complementary strategies for decreasing diarrhea morbidity and mortality: water and sanitation. Paper presented at the Pan American Health Organization, March 2-3. 9. Gould, J. & Nissen-Petersen, E. 2005) Rainwater catchment systems for domestic supply. Rugby: ITDG publishing. 25 10. Green, C. E. (2001). Can qualitative research produce reliable quantitative findings? Field Methods 13(3), 3-19. 11. Isabel Carter (2005). Encouraging good hygiene and sanitation. A PILLARS Guide. Tearfund. A company limited by guarantee. Regd in England No 994339. Registered Charity No 265464. 12. Laurent, P. (2005) Household drinking water systems and their impact on people with weakened immunity. MFS-Holland, Public health department. Online Available at: http://www. who. int/household_water/research/HWTS_impacts_on_weakened_immun ity. pdf 13. McKee, Neill (1992).

Social mobilization and social marketing in developing communities: Lessons for communicators. Penang: Southbound. 14. Nichter, M. (1993). Social science lessons from diarrhea research and their application to ARI. Human Organization 52(1), 53-67. 15. Ouagadougou: Ministere de la Sante du Burkina Faso. Curtis, V. A. , Cairncross, S, Yonli, R. (2000) Domestic hygiene and diarrhoea, pinpointing the problem. Tropical Medicine and International Health 5(1):22-32. 16. Pru? ss, A. , Kay, D. , Fewtrell, L. & Bartram, J. (2002). Estimating the global burden of disease from water, sanitation, and hygiene at the global level. Environmental Health Perspectives 110(5), 537–542. 17.

Ryan, M. A. K, Christian, R. Wohlrabe, J. (2001). Hand washing and respiratory illness among young adults in military training. American Journal of Preventive Medicine 21(2):79-83. 18. Saade, Camille, Bateman, Massee, Bendahmane, Diane B. (2001). The story of a successful public-private partnership in Central America: Handwashing for diarrheal disease prevention. Arlington, BASICS, EHP, UNICEF, USAID and World Bank. 19. UNICEF (2000). Learning from experience: Evaluation of UNICE’s water and environmental sanitation programme in India, 1966-1998. New York, UNICEF Evaluation Office, Division of Evaluation, Policy and Planning. 26 20. Verma, B.

L. & Srivastava, R. N. (1990). Measurement of the personal cost of illness due to some major water-related diseases in an Indian rural population. International Journal of Epidemiology, Vol. 19, No. 1: 169-175. 21. Water Quality and Health Councils (2010) Water storage tips to assist in emergency preparedness. Online Available at: http://www. waterandhealth. org/drinkingwater/water_storage. php3 22. WHO (World Health Organization) (2008a) Safer water, better health – Costs, benefits and sustainability of interventions to protect and promote the health. Online Available at: http://whqlibdoc. who. int/publications/2008/9789241596435_eng. pdf 23.

WHO (World Health Organization) (2008b) Guidelines for drinking-water quality- Third edition Incorporating the first and second addenda. Online Available at: http://www. who. int/water_sanitation_health/dwq/fulltext. pdf 24. WHO(2002). Water Supply. Environmental Health in Emergency. Online Available at: http://www. who. int/water_sanitation_health/hygiene/emergencies/em2002chap7. pdf 25. WHO/UNICEF (2005). Water for Life: Making it happen. http://www. who. int/water_sanitation_health/waterforlife. pdf . 26. WHO & UNICEF (2006). Meeting the MDG Water and Sanitation Target: The Urban and Rural Challenge of the Decade, WHO, Geneva and UNICEF, New York. 27. WSSCC (2004).

The Campaign: WASH Facts and Figures. Online Available at: Online Available at: http://www. wsscc. org/dataweb. cfm? edit_id=292&CFID=13225&CFTOKEN=70205233. 28. Wijk, Christine van (1998). Gender in water resources management, water supply and sanitation: Roles and realities revisited. Technical paper No. 33-E). The Hague: IRC International Water and Sanitation Centre. 29. http://www. unicef. org/media/files/JMPreport2012. pdf: UNICEF, WHO: Progress on Drinking Water and Sanitation update 2012 UPDATE. 27 30. http://www. cdc. gov/healthywater/global/wash_statistics. html : Centre of Disease Control and Prevention (2012) Global WASH Fast Facts 28

Image description. Better Health Channel logo End of image description. Handwashing – why it’s important A number of infectious diseases can be spread from one person to another by contaminated hands, particularly gastrointestinal infections, influenza and hepatitis A. Washing your hands properly can help prevent the spread of the organisms that cause these diseases. Some forms of gastroenteritis can cause serious complications, especially for young children, the elderly, or those with a weakened immune system. Drying your hands properly is as important as washing them.

Ima ge des crip When to wash your hands You should wash your hands thoroughly: • • • • • • • • • Before preparing food Before eating Between handling raw and cooked or ready-to-eat food After going to the toilet or changing nappies After smoking After using a tissue or handkerchief After handling rubbish or working in the garden After handling animals After attending to sick children or other family members. How to wash your hands properly To wash hands properly: • • • • • • • • • • Wet your hands with warm water.

Apply one dose of liquid soap and lather (wash) well for 15–20 seconds (or longer if the dirt is ingrained). Rub hands together rapidly across all surfaces of your hands and wrists to he lp remove dirt and germs. Don’t forget the backs of your hands, your wrists, between your fingers and under your fingernails. If possible, remove rings and watches before you wash your hands, or ensure you move the rings to wash under them, as microorganisms can exist under them. Rinse well under running water and make sure all traces of soap are removed, as residues may cause irritation.

Pat your hands dry using paper towels (or single-use cloth towels). Make sure your hands are thoroughly dry. Dry under any rings you wear, as they can be a source of future contamination if they remain moist. Hot air driers can be used but, again, you should ensure your hands are thoroughly dry. At home, give each family member their own towel and wash the towels often. Handwashing – why it’s important Page 1 of 4 How to wash and dry hands with liquid soap and water (Click to open larger image) Use warm water

Cold water is better than no water at all for a one-off hand wash, but should not be used for routine handwashing. Soap lathers (soaps up) better with warm water. The active ingredients on the surface of the soap are released more easily, making them more effective in cleaning your hands of dirt, grease and oils, without stripping away the natural oils in your skin. Using cold or hot water can also damage the skin’s natural oils. Over time, this can cause dermatitis. Soap is important Soap contains ingredients that will help to: • • • Loosen dirt on your hands.

Soften water, making it easier to lather the soap over your hands. Rinse your hands, leaving no residues to irritate and dry your skin. Soaps can have different pH – they may be neutral, slightly alkaline or slightly acidic. That’s why some soaps irritate some people and not others. Perfumes in soap can be another reason why some people have skin reactions. Liquid soap is best Handwashing – why it’s important Page 2 of 4 Generally, it is better to use liquid soap than bar soap, particularly at work.

The benefits of liquid soap include: • • • • Hygiene – it is less likely to be contaminated. Right amount – liquid soap dispensers do not dispense more than required (more is not better). Less waste – it’s easier to use, with less wastage. Drop-in cassette dispensers use all the soap. Saves time – liquid soap dispensers are easy and efficient to use. Disposable liquid soap cassettes are convenient, as you do not have to wash and thoroughly dry the refillable container before refilling. If you want to use refillable cont

Background Oil spills are horrible they damage the environment and may destroy ecosystems. This question has puzzled most scientists and elementary grade kids for a long time : What’s the best way to remove oil from water? Lets bump it up to using Marvel Mystery oil ,which is a Motor oil brand, so we have a nice bright red color. There are 3 main ways to separate oil from water and 3 absorbents that can be used.

The first way to remove oil is A belt, hose or disc, moves through a layer of oil, which causes the oil to adhere to the surface and be carried off. The second way is to Coalesce which is when smaller oil drops in an oil/water mixture will separate slowly or not at all. Coalescing media is placed in an oil separator to catch and accumulate the finer oil drops then merge them into larger drops that separate quickly.

The third way is where the three absorbents come in which is Gravity separation, which is a fancy way of saying the oil and water layers are prominent. Two of three of the absorbents are fairly fast at removing oil. The third, well helps decompose the oil faster. The first absorbent is human hair, most people can see why because it grasp onto oil and keeps it but that way is kind of messy. The second absorbent is Saw dust which is a little cleaner then the hair but just about as messy.

The third way which dose not really absorb as much as decompose the oil is using pure nitrogen and phosphorus to form little Tar balls which aren’t as deadly to the environment as the liquid crude oil. But no there is a forth absorbent let to be endorsed by the government. It is a polymer called WENV-250 and it was developed by a scientist to remove oil more defiantly. The polymer is non toxic so it may be used to put on birds feathers then simply pulled off.

Rocky Shore Ecology: Holbeck Beach This study was conducted to deduce whether the height to width ratio of limpets altered across the three main zones on the shore: upper, middle and lower. It was carried out on Holbeck Beach, North Yorkshire, where limpets were measured in all three zones using random sampling. We found a significant difference in the height to width ratio between the upper and lower shore and upper and middle shore. This is due to many factors, including the threat of desiccation and strong waves. Limpet Patelle VulgataRocky ShoreEcologyHolbeck Beach

Upper ShoreMiddle ShoreLower ShoreCallipers QuadrentRandom SampleDesiccation INTRODUCTION Common limpets, Patella Vulgata, are found, on rocky shores, wherever there is an area firm enough for attachment on rocks, stones and in rock pools. [1] The common limpet is commonly found on Holbeck shore in relatively high abundance. Patella vulgata are in the taxonomic group gastropoda, and the family acmaeidae. They are abundant on rocky shores of all degrees of wave exposure but a high density of seaweed makes it harder for the Patella Vulgata to attach itself to the rocks, so limpet density is reduced. 2] Patella Vulgata have the ability to use their mucus and their ‘foot’ to clamp down upon the rock with considerable force. This allows them to remain safely attached at all times, despite strong wave action and the threat of desiccation during low tide. When the limpet is fully clamped onto the rock it is almost impossible to remove them. The common limpet is a temperate species, so is found mainly across Europe, spread from Norway to Portugal. The grey conical shell of Patella Vulgata can reach a width of 6cm and height of 3cm with ridges radiating from the central apex.

The muscular foot of the limpet is usually a yellow colour and attached to the smooth interior of its shell. [3] Limpets graze upon algae, which grows upon the rocks where they live. They can scrape the algae with its radula (a tongue coated with many rows of teeth) as they slowly move across the rock surfaces. The Patella Vulgata always return to the same spot, known as the homing scar, before the tide withdraws. The shells grow to match the contours of the rock in order to form a strong seal, protecting them from desiccation and also predation.

They find their way back to the same spot by using chemical cues, finding their own mucus track and following it back to their home point. [4] Limpets are the prey of a variety of creatures, including seals, fish, shore-birds, starfish and humans. The limpets have two defences: fleeing or clamping down to the rock. They can determine which would be the most effective by detecting chemicals in the environment. Patella Vulgata have the general lifep of 10 years but this can be drastically changed by the rate of growth. If there is an excess of food, the limpets grow exceedingly quickly but generally only live for around 3 years.

However, if food is sparse, limpets usually grow very slowly but can live up to 20 years. [5] Patella Vulgata are hermaphrodites and undergo a sex change during their life. At around 9 months they mature as males, but after a couple of years they change sex and become female. Spawning occurs annually, usually during the winter months as it is triggered by rough seas, which disperse the eggs and sperm. [6] The larvae has a pelagic life of about 2 weeks and then settles on rocks at a shell length of about 0. 2 mm, usually in rock pools or areas that are constantly damp. HYPOTHESIS

Our hypothesis states that there will be a difference in the height to width ratio of limpets on different parts of the beach: lower, middle and upper. The null hypothesis states that there will be no difference between the height to width ratio of limpets on different parts of the beach: lower, middle and upper. METHOD We visited an exposed rocky shore at Scarborough in order to deduce whether the height to length ratio of limpets changed across different zones of the shore. Initially, we had to identify the different area of the beach and we did this by using different types of seaweed and levels of diversity as an indicator. 7] The upper zone, also known as the high tide zone, does not have enough water to sustain large amounts of vegetation. [8] The predominant organisms are anemones, barnacles, hermit crabs and limpets. The rock pools in this area are inhabited by large seaweed and small fish. The middle shore, or middle tide zone, is submerged by water for approximately half of the cycle. This means that there is the capability to support much more marine vegetation, specifically seaweeds. The organisms found there are more complex and larger in size than further up the shore. 9] The rock pools can provide a suitable habitat for small fish, sea urchins, shrimps and zoo plankton. This area is more diversified than the upper shore. The lower shore, or low tide zone, is mostly submerged underwater. The most noticeable difference of this sub-region is the large diversity of different types of seaweeds. Organisms found in this zone are generally less adapted to periods of dryness. The creatures are generally the largest and most complex organisms on the shore as there are more sources of food as marine vegetation flourishes.

The way that we sampled was random, meaning that every point is equally likely to be selected, and selection of one point does not change the probability of including any other point. [10] Once we had deduced the zones, we picked a random point in the zone, which was always the origin, and used a random number chart to decide how we far would walk before putting down the quadrant. We then measured the height and width of all the limpets inside the quadrant. In order to measure the limpets accurately we used a set of callipers; the callipers were positioned from the posterior end of the shell to the anterior.

On average we measured 27 limpets per zone. We did not calculate a running mean but we knew from other students that variation decreases in the region of 20-25. We collected the data using a simple tally, adding to it during the day. RESULTS Lower to Middle Shore My hypothesis was that there would be a significant difference in the height to length ratio of limpets found between the lower and mid shore. My statistical test gave a t-value of 1. 3 at 52 degrees of freedom. This value is not large enough to give me any confidence that there is a statistically significant difference, as it is below 1. 8; therefore I must reject my hypothesis and accept my null hypothesis that there is no significant difference between the height to length ratio of limpets found between the lower and mid shore. Middle to Upper Shore My hypothesis was that there would be a significant difference in the height to length ratio of limpets found between the mid and upper shore. My statistical test gave a t-value of 3. 3 at 50 degrees of freedom. This value is larger than 2. 70, giving me a 99% confidence that there is a statistically significant difference and therefore I am able to accept my hypothesis. Upper to Lower Shore

My hypothesis was that there would be a significant difference in the height to length ratio of limpets found between the upper and lower shore. My statistical test gave a t-value of 3. 2 at 52 degrees of freedom. This value is again larger than 2. 70 giving me a 99% confidence that there is a statistically significant difference and therefore I am able to accept my hypothesis. CONCLUSIONS The data we collected shows that the smallest limpets found on the Holbeck shore were on the middle shore. We found that, on the lower shore, the limpets had the smallest height but the largest width.

This can be explained as they are the affected most by strong waves and are most at risk of being washed away. To combat these problems they have a shallow but wide shell to give the largest area for the muscular foot to hold onto the rock. Also, the limpets at the bottom of the shore are underwater for the longest time. This means that they have less problems combating desiccation than limpets in the other zones. This allows for a larger circumference of the shell, as it is not as essential to have a perfect seal to the rock. [11]

On the upper shore, the limpets we found were generally taller with a smaller base of their shell. Being far up the beach, they do not have as many strong waves which may wash them from their rock so they do not need as large an area for the muscular foot to grip the rock. However, the smaller circumference lowers the chances of having an imperfection in the shape of the shell compared to the homing scar, meaning that a perfect seal will be created. This is of paramount importance as they spend most of their time exposed and in the sunlight – meaning desiccation could easily occur. 12] To help prevent desiccation, the tall shell allows water to be trapped inside, creating a small pool and allowing the limpet to survive whilst being out of water during low tide. FURTHER DISCUSSION Apart from the threats of desiccation and strong waves, there may be other factors which influence the growth of limpets in different areas on the shore. One factor would be the feeding time available for limpets. [13] Limpets further down the shore spend more time underwater so they have more time to graze where as the limpets further up the shore have very little time under water so have very little grazing time. 14] The amount food eaten may in some way affect the growth patterns and development of the conical shells. Another factor is salinity. When the shore is submerged regularly by sea water, the salinity generally remains stable. However, in areas with an abundance of rock pools may have varied salinity levels. As rock pools constantly dry out, due to exposure to warm temperature, the water evaporates leaving behind the salt. [15] Overtime the salinity levels become high, making the rock pool inhabitable to many creatures, including limpets which can only tolerate normal sea water salinity. 16] Fluctuations in salinity alter the water potential and may cause cells in the limpet to become turgid or flaccid – both of which are dangerous. REFERENCES [1] – RG Evans (1974). Biology of British Limpets: Page 411. Found on 12. 10. 12. [2] – I Cockcroft. Website: Gyllybeach, Common Limpet. Found on 13. 10. 12. [3] – Fish, J. D. & Fish, S. (1996) A student’s guide to the seashore. Second Edition. Cambridge University Press, Cambridge. Found on 13. 10. 12 [4] – Website: pznow, Limpets. Found on 14. 10. 12. [5] – Hill, J. M. , (2000). Patella vulgata.

Common limpet. Marine Life Information. Found on 17. 10. 12 [6] – BBC (2005) Website: BBC, Science and Nature, Animal Fact Files, Common Limpet. Found on 12. 10. 12. [7] – Vipera, T. Website: Life Under The Sea, Zonation. Found on 18. 10. 12. [8] – YPTE (2010). Website: ypte, Environment, Rocky Shore Ecology. Found on 15. 10. 12. [9] – Rothery M (2005) Rocky Shore Handout. Found on 20. 10. 12. [10] – R Easton, Hall J. Website: Stats Glossary, Sampling. Found on 18. 10. 12. [11] – Norfolk WT (2011). Website: Norfolk Wildlife Trust, Wildlife, Common Limpet. Found on 20. 0. 12. [12] – J Adams (1999) “A Comparison of Width and Height of Common Limpets Between a Sheltered Shore and an Exposed Shore.. Found on 20. 10. 12. [13] – Levinton J. S. (1995) Marine biology: function, biodiversity, ecology. Oxford University Press. Found on 22. 10. 12 [14] – Bennett, I. (1987) W. J. Dakin’s classic study: Australian Seashores. , Angus & Robertson, Sydney. Found on 22. 10. 12. [15] – Wars (2011). Website: Limpet Wars, marine science. Found on 20. 10. 12. [16] – Knox G. A. (2001) The ecology of seashores. CRC Press. Page 557. Found on 20. 10. 12

Meghann Kiphart AP Biology Lab Report Number One Mrs. Irvine Introduction: Because all molecules have kinetic energy and are constantly in motion cells go through a process called diffusion. Diffusion is the movement if molecules from an area of higher concentration to and area of lower concentration. This process with continue to occur until an equilibrium is reached. is a different and unique kind of diffusion. Osmosis is the diffusion of water through a permeable membrane. The phrase “permeable membrane” means that the membrane will only allow specific molecules through such a water or oxygen.

In Osmosis water will travel from an area of higher water potential or an area of lower water potential. Hypothesis: I think that in this lab, osmosis and diffusion will occur between the solutions of different concentrations until a equilibrium is reached and there is no movement of water. Materials: EXERCISE 1A: Diffusion The materials include a 30-cm piece of 2. 5-cm dialysis tubing, 15-mL of the 15% glucose/1% starch solution, 250-mL beaker, distilled water, 4-mL Lugol’s solution, and string. EXERCISE 1B: Osmosis The materials used include 25-mL of these solutions: distilled water, 0. M sucrose, 0. 4M sucrose, 0. 6M sucrose, 0. 8M sucrose, and 1. 0M sucrose, scissors, string, a balance, six 250-mL cups, and six 30cm strips of dialysis tubing. EXERCISE 1C: Water Potential The materials that were used included 50mL of distilled water, 0. 2M sucrose, 0. 4M sucrose, 0. 6M sucrose, 0. 8M sucrose, and 1. 0M sucrose, six 250mL cups with lids, 4 potato cores for each cup, a balance, and paper towel. EXERCISE 1D: Calculation of Water Potential from Experimental Data This exercise required a calculator and a pencil. Procedure: EXERCISE 1A:

Soak the dialysis tubing in water before you start the experiment. Tie off one end of the tubing to form a bag like structure. Through the open end of the bad, place the starch solution in to the bag. Tie off the other end of the bag to secure the substance inside. Make sure to record the color of the solution in Table 1. 1. Next you’re going to text the starch solution for the presence of glucose. Record the results in Table 1. 1. Fill a 250ml cup about 2/3 of the way full with distilled water. Add 4ml of Lugol’s solution into the distilled water. Record the color of the solution in the Table 1. . Put the bag in the cup full of the solution. Allow the bag and cup to stand over night. The next day record the final color of the solution in Table 1. 1. Finally test the liquid in the cup and in the bag for the presence of glucose. Record the final results in Table 1. 1. EXERCISE 1B: Get six strips of presoaked dialysis tubing and create a bag like was shown in exercise 1A. Pour 25mL of the six solutions into each of the six bags. Tie off the other end of the bags. Rinse each bag gently with distilled water and dry the outside of the bag with a paper towel.

Weigh each bag and record the results in Table 1. 2. Put each of the six bags into the cups with the six different solutions. Let stand over night. The next day remove the bags from the water and carefully dry the bags with paper towel. Weigh each bag and record them in Table 1. 2. Gather the other lab group’s data to be able to complete Table 1. 3. EXERCISE 1C: Pour 50mL of the solutions into a labeled 250mL cups. Using a cork borer, cut the potato into 24 cylinders. (4 potato cores x 6 cups = 24 potato cores altogether) weigh the mass of each set of 4 potato cores.

Record the data in Table 1. 4. Put 4 potato cores into each solution cup. Cover the cup with a lid to prevent evaporation. Let stand overnight. Remove cores from the cup and dry them with a paper towel. Then determine there combined weigh in groups of 4 (from the same cup). Record the results in Table 1. 4. Calculate the percentages changes in mass. Collect the class data and determine the class change in mass. EXPERIMENT 1D: Determine the solute, pressure, and the water potential of the sucrose solution. Then, graph the information that is given about the zucchini cores. Questions:

EXPERIMENT 1A: 1. Which substances are entering the bag and which are leaving the bag? What evidence supports the answer? Distilled water and IKI are leaving and entering. Glucose is able to leave the bag. 2. Explain the results that were obtained. Include the concentration differences and membrane pore size in the discussion. Glucose and small molecules were able to move through the pores. Water and IKI moved from high to low concentration. 3. How could this experiment be modified so that quantitative data could be collected to show that water diffused into the dialysis bag?

You could mass the bag before and after it is placed into the solution. 4. Based on your observations, rank the following by relative size, beginning with the smallest: glucose molecules, water molecules, IKI molecules, membrane pores, and starch molecules. Water molecules, IKI molecules, Glucose molecules, membrane pores, and starch molecules. 5. what results would you expect if the experiment started with a glucose and IKI solution inside the bag and only starch and water outside? The glucose and IKI would move out of the bag and turn the starch and water solution purple/ blue.

The starch couldn’t move inside the bag because its molecules are too big to pass through the membrane of the tubing. EXERCISE 1B: 1. Explain the relationship between the change in mass and the molarity of sucrose within the dialysis bags. The solute in hypertonic and water will move into the bag. As the molarity increases the water moves into the bag. 2. Predict what would happen to the mass of each bag in the experiment of all the bags were places in a 0. 4M sucrose solution instead of distilled water. With the 0. 2M bag, the water would move out. With the 0. M bag, there will be no net movement of water because the solutions reach equilibrium. With the 0. 6M-1. 0M bags the water would move into the bags. 3. Why did you calculate the percent change in mass rather then simply using the change in mass? This was calculated because each group began with different initial masses and we would have different data. All the groups need consistent data. 4. A dialysis bag is filled with distilled water and then places in a sucrose solution. The bag’s initial mass is 20g and its final mass is 18g. Calculate the percent change of mass, showing your calculations. 18-20)/20) x 100 = 10% 5. The sucrose solution in the cup would have been hypotonic to the distilled water in the bag. EXPERIMENT 1D 1. If the potato core is allowed to dehydrate by sitting in the open air, would the water potential of the potato cells decrease or increase? Why? It would decrease because the water would leave the cells and cause the water potential to go down. 2. If a plant cell has a lower water potential then its surrounding environment and if pressure is equal to zero, is the cell hypertonic or hypotonic to its environment?

Will the cell gain water or lose water? It is hypotonic and it will gain water. 3. The cup is open to the atmosphere, what is the pressure potential of the system? The pressure potential is zero. 4. Where is the greatest water potential? In the dialysis bag. 5. Water will diffuse out of the bag. Why? It is because the water moves from the area of high water potential to an area of lower water potential. 6. What effect does adding solute have on the solute potential component of the solution? Why? It makes it more negative 7.

Consider what would happen to a red blood sell placed in distilled water: A) which would have the higher concentration of water molecules? Distilled Water B) which would have the higher water potential? Distilled Water C) what would happen to the red blood cell? Why? It would leak, because it would take to much water. Conclusion: In Exercise 1A the data collected helped tell which molecules can and can not move across a cell membrane. IKI, we know because of its color change, was able to move across a membrane. Starch, although, is too large to move across a membrane.

Glucose was able to move freely, along with the water, across the cell membrane. In 1B, it was proven that water moves faster across the cell membrane then sucrose. The water moved to help reach equilibrium between the 2 solutions. The sucrose molecules are too big to move across the membrane as fast as water can. In experiment 1C showed that the potatoes contained sucrose. The sucrose in the potato raised the solute potential, which lowered the water potential. The cup of distilled water had a high water potential water moves down the concentration gradient, causing the potato cores to take on water.

I. Problem/Question

There is currently an increasing commercial viability of mud crabs especially in subtropical countries. However, due to seasonal factors affecting the spawning of these species, there a need to develop methods for “year-around larval production” (Zeng, 2007, p. 1478-1479) arises. There are no current techniques that would enable this process. Therefore, this study investigated on the feasibility of out-of-season mud crab spawning induction. It also determined whether in vitro incubation of eggs is possible.  An evaluation of the temperature effects on the rates of embryonic development of mud crabs was also conducted. This is in order to provide predicted dates of hatching for berried mud crab females (Zeng, 2007, p. 1478-1479).
II. Experimental procedures/Method

The researchers kept female individuals of S. paramamosain in 1000L tanks. These holding tanks contained seawater, filtered with sand, with constant salinity of 29-32 g/L but with uncontrolled temperature between 10-30 °C.  In order to determine the “proximate maturation index (PMI)” (Zeng, 2007, p. 1479), crab ovarian development was regularly checked every fortnight using a calliper to measure the width of the strip of light in the carapace when shone with bright light from underneath (Zeng, 2007, p. 1479).

The induction experiment was started with the random selection of mature subjects with <2mm PMI. The researchers removed their eyestalks and were moved to indoor aquaria, each provided with sand trays as egg attachment substrate, with controlled environmental temperature and photoperiod. The aquaria were regularly siphoned for sanitary maintenance. The researchers proceeded with testing their first objective, “in vitro egg incubation and effects of temperature on egg development” (Zeng, 2007, p. 1480).

Setups with 200 eggs were used for the evaluation of temperature effects on development of the embryo. These were incubated respectively at 10, 15, 25, 27, 30, and 35 °C ± 0.5°C, with each treatment having triplicates. After acclimatization, embryonic development of the eggs in each treatment was monitored. The eggs were examined under the microscope in a regular time interval during the first 36 hours of the incubation. Then the larvae were attempted to be cultured in vitro, testing whether they are able to “reach the first juvenile crab stage” (Zeng, 2007, p. 1480).

III. Experimental Results

Between the months of November and April, 90% of female mature crabs with ablated eyestalks successfully spawned. The remaining 10% was due to the mortality of the individual. Newly extruded crab eggs were observed to “assume an oval shape” while some others were dented on a side, with undistinguishable outer and inner membranes. But eggs later transform into a spherical shape with distinct membranes (Zeng, 2007, p. 1481).

Eggs at 35 °C resulted were characteristically asymmetric and unsynchronized, relative to the ones in 25-35°C treatments. Such abnormal cellular division were also manifested by eggs in temperature treatments between 10 and 35 °C. The embryonic development of eggs incubated at 15 °C was observed to have arrested at the gastrula stage on the 32nd day, followed by the termination of the experiment.

Eggs incubated in vitro successfully hatched in temperatures between 20 and 30 °C. The incubation period was reduced by 14 days with incubation temperature increase from 20 to 25 °C. The researchers divided the embryonic development of the mud crab into 10 stages. They characterized the different morphological attributes of the various stages, revealing different temperature effects on embryonic developmental rates of each stage (Zeng, 2007, p. 1481).

IV. Conclusions/Summary

The researchers were able to spawn the female mud crabs during the non-season period. They were successful in rearing crabs to their juvenile stage with dry weights comparably the same with naturally hatched eggs. They found that temperature has varying effects on embryonic developmental stages. This information enabled them to make accurate predictions of hatching of female mud crab eggs (Zeng, 2007, p. 1481-1482).
V. Discussions

Spawning mud crabs during off season months was made possible through eyestalk ablation and increased water temperature. This is indeed a very important finding as it allowed scientists to provide farmers an improved method in acquiring their preferred harvest of mud crabs. The success of spawning induction is based on the development of gonads during colder seasons and their ability to spawn at higher temperature seasons. Just by elevating the temperature levels, mud crab females already perceive this as a stimulus for them to carry out spawning (Zeng, 2007, p. 1483).

Since this industry has promising commercial potentials, it is essential to develop cost efficient methods that would accommodate high yields. In this study, the cost of maintaining numerous berried crabs would be reduced through in vitro incubation of crab eggs. These can basically be placed in either aeration-generated water or in static water. As researchers were able to determine the hatching temperature for mud crabs, farmers are now enabled to determine when the hatching schedule would be.

This would allow them to obtain juvenile crabs at any time that they please regardless of the season. The abnormality observed in the cellular division of mud crab embryo in certain temperatures are proposed to be adaptive mechanisms to enable mud crab larvae to have a scheduled hatching during warmer seasons when they can have enough access to food such as plankton (Zeng, 2007, p. 1484).

INDUS WATER TREATY OF 1960 by William H. Thompson [February 2013] The Indus Water Treaty (IWT) of 1960 is an example of a mutually beneficial conflict or, as Kriesberg and Dayton would define it, a constructive conflict. Born of the dissolution of the British Crown Colony of India in 1947, the treaty recognized the mutual needs of India and Pakistan, and the necessity of ensuring continuing access to the waters of the Indus River System for both nations.

Although the treaty has survived “two and a half wars and frequent military mobilizations” as well as a nuclear arms race, current moves by both Pakistan and India regarding dispute mediation threaten to dissolve the treaty. Differences in interpretation, Pakistani mismanagement of its own water resources and the ongoing question of the status of Kashmir each threaten the continued observance of the treaty. Neither nation can afford the loss of this treaty. For each nation this treaty has been a source of ongoing diplomatic relations, requiring annual meetings and open verification of water projects within the covered regions.

It has been used to address non-water issues and to placate each other in times of crisis. It has also ensured that water continues to flow between the two, in spite of the strategic advantage that India could gain by stopping that flow. This paper will outline some of the dangers affecting the future of the IWT. It will address the interpretation of treaty clauses by neutral parties and how that has resulted in diplomatic escalation by Pakistan. It will address the very real concern for Pakistan that India has the superior strategic position with regard to control of the Indus System.

It will also highlight the inadequate water infrastructure within Pakistan and the affect that this has on the ability of India to complete its own water projects. The paper will describe certain indicators of the health of the treaty. Finally, it will outline two scenarios for the future of the IWT and the likely outcome of each. The goal of addressing these issues is to stress the importance of this treaty over national concerns for control of water and how the mutual control of the Indus system is the best solution for both nations.

Before exploring the continued existence of the Water Treaty of 1960, and the potentially far reaching effects of its nullification, it is necessary to provide a brief history of the Indo-Pakistani conflict, especially as it relates to the Kashmiri region and control of the Indus River System. When the British Parliament passed the Indian Independence Act of 1947, its primary concern was achieving a speedy settlement of the partition rather than the stability of the resulting entities.

Sir Cyril Radcliffe, the English barrister charged with partitioning the Indian colony into two separate entities, arrived in New Delhi on 8 July 1947 to learn that the date of independence for both newly formed nations of India and Pakistan had already been set for 15 August of that same year. The rules for the partition of India and Pakistan, established in negotiations between the British representative Lord Mountbatten, the Indian National Congress representative Jawaharlal Nehru and the Muslim League representative Muhammed Ali Jennah, focused the division along religious lines.

In certain provinces with no clear religious majority, most notably those bordering Punjab and Bengal, the citizens of the province were to be given the opportunity to vote over which country to join. Independent princedoms, such as Kashmir, were given the option of joining with either state, but were encouraged to hold a plebiscite if the desires of the people were in doubt. The resulting boundaries would have three far-reaching results.

First, the sudden change in citizenship (from nominally British to Pakistani or Indian respectively) resulted in bloodshed and mass-exodus as Muslims moved from India to Pakistan and Hindus moved to India from Pakistan, as well as an almost instantaneous nationalism within both nations. Second, when establishing borders between the states it did so with little regard to natural boundaries, such as rivers, and little thought to allocation of the infrastructure and resources now shared by the two states.

What had been created by one central government, such as irrigation systems, canals, and dams, was now controlled by two with no standing agreement over how they should be shared. Finally, in giving the rulers of independent princedoms the right to choose which country to join, the prince was expected to abide by the wishes of his subjects; in the case of Kashmir, the prince made his own choice. Common sense should have dictated that the province becomes the northernmost province of Pakistan: Its people were predominantly Muslim and it controlled the flow of the Indus River into Pakistan.

Kashmir as a province of Pakistan was likely the vision of the British, Muslim and Hindu negotiators of the partition. Unfortunately, the status of the various princedoms, including Kashmir, was left to each ruling prince. Although not alone in originating the Indo-Pakistani conflicts, the decision of Hari Singh, the Maharaja of Kashmir, to join India rather than Pakistan has played a vital role in exacerbating them. One oddity of the partition of the former British colony is the Standstill Agreement.

This agreement stated that the flow of the Indus between East and West Punjab (India and Pakistan) would remain at the same level from the date of partition until 31 March, 1948 and that Pakistan would pay a set fee for the water that flowed. As Pakistani forces crossed the border of Jammu and Kashmir to protect Muslims and Indian forces were airlifted into Kashmir to defend India’s territorial boundaries, the dams, canals and barrages along Indus tributaries continued to operate and adjust flows to ensure that water reached the fields of Pakistan.

And, as these things occurred, Pakistan continued to pay its water fee to India. However, on 01 April, 1948, with the agreement ending and no new agreement in place, the flow of water stopped. Although India and Pakistan would agree to a resumption of water deliveries, two precedents had been set: Pakistan recognized that it was in an untenable position and India had demonstrated that it would abide by existing agreements but, in the absence of agreement would act in its own best interests.

In 1952, the World Bank offered to mediate the dispute over Indus Waters. The resulting treaty, based on the water usage needs of each, water availability in the Indus System and mutual development of the watershed granted India the use of several rivers flowing through Kashmir for power generation, but stipulated that the usage must allow free flow of the waters into Pakistan. Each nation must announce water development plans and allow for the inspection of these projects by engineers from the other nation.

It established a Permanent Indus Commission, made up of engineers from each nation, which would meet annually to discuss development issues and treaty implementation and established steps for dispute arbitration. Modern interpretation of the provisions of a treaty established in 1960 have strained the agreement and resulted in an escalation of Pakistan’s arbitration demands. Until 2005 all disputes over water projects had been resolved through the annual meetings of the Permanent Indus Commission. This changed with Indian plans to build the Baglihar Dam, a hydroelectric project, across the Chenab River.

Although planning began in 1992, Pakistani engineers first objected to the project in 1999 on the grounds that it blocked the free flow of water within the Indus System in violation of the IWT. India contended that, in spite of the fact that it did not comply with the original treaty, the design of the dam was sound and that it would not only allow for the flow of water but would ensure that water supplies were available throughout the year. Pakistan referred the dispute to the World Bank for neutral arbitration under terms of the IWT.

Although the neutral arbiter agreed in principal that the Indian project violated some aspects of the treaty, the violations were determined to be based on “sound and economic design and satisfactory construction and operation” and the project was allowed to continue. While Pakistan agreed to the decision of the World Bank, its next dispute, over the Kishanganga Hydroelectric Dam, was taken directly to the International Court of Arbitration. Although this level of arbitration is specified in the IWT, it is the first time that any dispute under the treaty has been taken to this level.

The fact that Pakistan skipped neutral arbitration in favor of the International Court may be a signal that it mistrusts the neutrality of the World Bank. Although the Court has not yet ruled on the project, a ruling in favor of India may convince Pakistan that the treaty is no longer in its best interests. The escalating arbitration demands of Pakistan reflect some concern over individual water projects, which was reflected in its arbitration request concerning the Baglihar Dam project, and more concern for the strategic implications of the Indian system as a whole.

As most agree, no single Indian project could shut down water supplies to Pakistan. However, there is general agreement that India holds the superior position regarding control and usage of the Indus River. And there is agreement that the sheer number of dams along the northern Indus System could indeed have adverse effects on the water available to Pakistan. While Indian water needs are fulfilled by three rivers, the Ganges, the Brahmaputra as well as the Indus,

Pakistan is served almost exclusively by the Indus, over which India maintains control. Although India contends that it has never diverted water from Pakistan, the water stoppage of 1948, when East Punjab halted water flow into West Punjab, is ever present in Pakistani strategic thought. India has the greater GDP, and therefore a greater ability to withstand delays to its water projects, and a larger military, so it cannot be easily intimidated into acceding to Pakistani demands.

As Pakistani negotiators have stated, the Indian negotiating strategy is “one of delay, of foot dragging, of ‘tiring you out’;…of “creating facts”, proceeding with construction plans, even when aware that the plans might well violate the treaty, so that Pakistan, confronted eventually with fait accompli, would have no choice but to cut its losses and accept an unfavorable compromise settlement; and … insisting on a bilateral framework of talks, without intending ever to settle on any but India’s terms. Although Pakistani negotiators may believe that India can drag negotiations on, the reality is that each referral to arbitration has put a great burden on India in time to completion. In the case of the Baglihar Dam, India announced its plans in 1992, began construction in 1999, the project was taken to arbitration in 2005 and the entire project was not completed until 2010. This case is similar to other projects which have taken 10+years from commencement, through negotiation, to completion.

Some, especially within Pakistan, have suggested that the treaty is no longer useful, that it is too strategically disadvantageous to Pakistan and that the only solution to the issue is to take control of Kashmir and the northern Indus System. Others have expressed concerns that India’s hydroelectric projects may force Pakistan to abrogate the treaty and spark a war over Kashmir and control of the Indus.

Whether concerns over war between the two nuclear nations are meant as a warning or a threat they have come often enough since the dispute over the Baglihar Dam that they must be seen as a real concern. With multiple Indian hydroelectric projects in the planning stage (although the actual number is in dispute), the opportunities for “hawks” within Pakistan to demand war will continue to place pressure on politicians and the military to accept nothing less than a halt to all projects.

The disputes over Indian projects have allowed Pakistan to divert attention away from its own weaknesses with regard to water availability. Although Pakistan often contends that Indian projects on the northern Indus have resulted in a loss of useable water within Pakistan, it is “a case of wastage and unequal distribution by internal forces” that has resulted in less water availability within Pakistan. This loss in water availability is due to aging transfer systems (pipes, canals), increasing silt levels within dams, corruption and inefficiency and low expenditure on water sector development.

Ninety percent of Pakistan’s irrigable water is supplied by the Indus; an aging system of canals, barrages and hydroelectric dams within Pakistan has resulted in waste within its own water management systems. This is largely a result of heavy sediment composition of the Indus. Water storage systems and canals have filled with sediment over time, resulting in less water availability and susceptibility to flooding, especially during heavy monsoonal rains. The IWT has been used as a means to, if not settle other non-water related disputes, to at least achieve a hearing of them, or to ease the tensions between the nations.

Most recently, in 2009, the Pakistan Commissioner of Indus Waters had been asked about developments on the Nimoo-Bazgo Hydro Project and whether his office had inquired about inspecting the development. His response was that “We would like to go there when the tension between India and Pakistan following the Bombay attacks ease. ” In the wake of the Mumbai attacks, the Pakistani official chose to delay his inspection to avoid inciting an already tense situation.

India had threatened to pull out of the treaty as a response to cross-border terrorism in 2001-2002, and has used its control of the upper Indus to exert pressure on Pakistan to halt attacks. Although this may be viewed as using its hegemonic power over water flows to exert pressure, the alternative is that war was avoided through the use of the existing treaty. Should either India or Pakistan see the treaty as having outlived its usefulness, the nations have two choices: nullification or renegotiation.

Renegotiation would be the most desirable choice for the nations and the region. Indeed, renegotiation of the treaty may be a necessity. Guarantees of water deliveries through the Indus system may be unsustainable if climate change models are correct. Pakistan is currently able to store only 30 days of water, leaving it highly vulnerable to even mild fluctuations in water flow. This vulnerability exists in a period when the Indus is at its highest flow in 500 years due to the melting of the Himalayan glaciers that feed the system.

The expectation, although the calculations differ, is that the flow will slow as the glaciers recede, leaving both India and Pakistan struggling for water. Signs that offers to renegotiate are real would have to include two things; 1. Renegotiation would have to be open to public scrutiny and third party mediation and 2. They would have to include cooperative agreements on joint water projects. Renegotiation of the treaty under these conditions would indicate that both parties are committed to the IWT in some form.

Nullification may be more difficult to predict. As stated above, the treaty itself has survived at least three and a half conflicts and terrorist incursions. Escalation of hostilities may not be a reliable indicator of nullification. The current escalation of arbitration demands under the current treaty may provide some warning, should Pakistan reject the findings of the current International Court arbitration. Although the current case was brought over the Indian Kishanganga dam, it is actually a story of two dams.

Pakistan is currently building a dam on the same river, the Neelam-Jhelum Dam. Should arbitration be decided in India’s favor, the Kishanganga dam will divert water away from the Neelam-Jhelum, making the dam useless. Should this occur and the two nations are unable to come to some accommodation, Pakistan may determine that the treaty is no longer in its best interest. Without the treaty its guarantees of water flow into Pakistan, the nation may see war as the only alternative. There are two likely scenarios for future developments with regard to the IWT.

The first is and most likely scenario is a renegotiation of the treaty. For renegotiation to occur, it would most likely need to be initiated by India, as such an offer would likely be seen by the Pakistani public as bowing to Indian pressure. In addition, were Pakistan to request a renegotiation, India most likely would have the upper hands in discussions. The catalyst for renegotiation would most likely be the ongoing demands for arbitration from Pakistan and the continuing delays in Indian construction projects.

In return for a greater freedom to build on the upper Indus, India would have to offer significant concessions, the most likely being the instigation of joint projects to ensure more efficient irrigation to Pakistani cropland and more effective flood mitigation. Should India successfully convince Pakistan that a new treaty would provide more favorable water availability and would result in less control over the Indus System by India, then the renegotiation could be both a diplomatic and public relations success.

The end result would be that both countries would be much better prepared should the flow of the Indus be reduced in the future. The second scenario is less hopeful and also less likely. Should Pakistan determine that the existing treaty is no longer in its best interest and it believes that Indian projects will result in less water availability on the Indus, Pakistan may nullify the treaty. In this case, war would be highly likely to occur as Pakistan attempts to seize control of Kashmir and the upper Indus River.

This scenario itself has three likely outcomes. 1. In order to avoid a nuclear war, the international community brokers a cease-fire. India retains control of Kashmir and effectively ends both Pakistan’s claims to the province and any obligations to allow the free flow of water to Pakistan. While Pakistan would still receive some flow, mainly as a result of flood control measures and sediment flushing from Indian dams, it would not be enough water to enable Pakistan to adequately irrigate or to provide fresh water to its people.

The aging irrigation infrastructure would continue to deteriorate, compounding an already untenable situation. The threat of nuclear war would hang over the region for the foreseeable future as radical elements within Pakistan are able to seize power and Pakistan becomes a failed, pariah state. 2. As a result of a brokered cease-fire, Kashmir achieves independence. Kashmir brokers its own water treaty with both India and Pakistan: India agrees to maintain the existing hydroelectric dams and water storage in return for continued access to the electricity being generated.

Pakistan continues to receive flow from the Indus River, but at lower levels than under the IWT as Kashmir diverts and stores some of the water for its own irrigation. Pakistan’s irrigation and storage systems continue to deteriorate, but at a less noticeable pace than under the first nullification scenario. Radical elements are able to achieve some power within Pakistan, but moderates are able to maintain control and because of the existing water treaty are able to contract assistance from China and the United States to upgrade irrigation and water storage.

Although still a nuclear power, Pakistan is unable to maintain parity with India on a military or economic level, effectively diminishing the threat of nuclear war. 3. Pakistan achieves strategic surprise and is able to seize control of Kashmir and the upper Indus River prior to the brokered cease-fire. Rather than increasing the flow of water to irrigate, Pakistan maintains the current hydroelectric systems built by India, selling some of the power to India and diverting the rest for its own use.

Pakistan fails to address its own interprovincial water sharing issues: In addition to existing squabbles between Punjab and Sindh, it has added Kashmir to the mix with its own demands for irrigation and fresh water. Although Pakistan is able to maintain water flow to support irrigation, it is below the level of the IWT. India and Pakistan continue their adversarial relationship but without the benefits of diplomatic exchange. Radicals within Pakistan are able to exploit the inequitable division of water between the provinces and, in spite of its Muslim majority, Kashmir never becomes a fully integrated part of Pakistan.

Because of its need to maintain both a military balance with India and to secure its facilities against domestic terror attacks, it is unprepared for the dropping water flow due to the recession of the Himalayan glaciers feeding the Indus. The region continues to be an international concern as China and the United States jockey for influence. Although the scenarios regarding a nullification of the IWT may be unduly negative, most academic studies agree that the Indus Water Treaty of 1960 is too important to regional relations for either India or Pakistan to seek an alternative.

Whether the treaty continues in its present form, which is increasingly unlikely, is renegotiated as part of a larger brokered deal, or is restructured according to some recognition of Indian responsibility to its neighbor, the treaty has survived an ongoing adversarial relationship for 53 years due to both its effectiveness and its utility. With the worldwide potential for resource scarcity, the potential exists that other nations sharing water resources could model their own disputes on the IWT, but only if Pakistan and India are able to resolve their own ongoing issues.